journal article·2023-05-08

Enhanced Probe Bonding and Fluorescence Properties through Annealed Graphene Oxide Nanosheets

Chong-You Chen, Hsien-Yi Chiu, Shing-Jyh Chang, Nai-Lun Yeh, Chia-Hao Chan, Chuan-Chi Shih, Shiue-Luen Chen, Jia-Wei Yang, Chien-Yu Huang, Guan-Yu Chen

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Abstract

Graphene oxide (GO) has been widely used in biological sensing studies because of its excellent physical and chemical properties. In particular, the rich functional groups on the surface of GO can effectively enhance the bonding of biomolecules and serve as an efficient sensing substrate. However, when biomolecules are labeled with fluorescence, the GO interface affects the biomolecules by reducing the fluorescence properties and limiting their applications in biosensing. Here, we establish an annealed GO (aGO) substrate through the annealing process, which can effectively increase the bonding amount of a DNA probe because of the accumulation of oxygen atoms on the surface without significantly damaging the nanosheet structure. Furthermore, we prove that the aGO substrate can effectively maintain its fluorescence performance and stability by exposing more graphic domains. Overall, this study successfully verifies that GO's interface annealing modification can be used as an alternative innovative interface application in biosensing.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Graphene and Nanomaterials Applications · Advanced biosensing and bioanalysis techniques · Graphene research and applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Biomedical Engineering, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu 300, Taiwan

Department of Electrical and Computer Engineering, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu 300, Taiwan

Department of Dermatology, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu 300, Taiwan

Department of Dermatology, College of Medicine, National Taiwan University, Taipei100, Taiwan

Department of Medical Research, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu 300, Taiwan

Department of Dermatology, National Taiwan University Hospital, Taipei 100, Taiwan

Gynecologic Oncology Section Department of Obstetrics and Gynecology, Hsinchu MacKay Memorial Hospital, Hsinchu 300, Taiwan

Department of Community and Family Medicine, National Taiwan University Hospital Hsinchu Branch, Hsinchu 300, Taiwan

Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 300, Taiwan

Funding

Ministry of Education · National Health Research Institutes · Ministry of Science and Technology, Taiwan · Council of Agriculture · National Taiwan University Hospital, Hsin-Chu branch · Higher Education Sprout Project, National Yang Ming Chiao Tung University · Hsinchu Mackay Memorial Hospital

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