DataVXACS Biomaterials Science & EngineeringPaper
journal article·2017-08-14Open access

Confined Sandwichlike Microenvironments Tune Myogenic Differentiation

José Ballester-Beltrán, Sara Trujillo, Enateri V. Alakpa, Vicente Compañ, Rafael Gavara, Dominic Meek, Christopher C. West, Bruno Péault, Matthew J. Dalby, Manuel Salmerón-Sánchez

ACS Biomaterials Science & Engineering· Volume 3· Issue 8
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Source abstract

Abstract

Sandwichlike (SW) cultures are engineered as a multilayer technology to simultaneously stimulate dorsal and ventral cell receptors, seeking to mimic cell adhesion in three-dimensional (3D) environments in a reductionist manner. The effect of this environment on cell differentiation was investigated for several cell types cultured in standard growth media, which promotes proliferation on two-dimensional (2D) surfaces and avoids any preferential differentiation. First, murine C2C12 myoblasts showed specific myogenic differentiation. Human mesenchymal stem cells (hMSCs) of adipose and bone marrow origin, which can differentiate toward a wider variety of lineages, showed again myodifferentiation. Overall, this study shows myogenic differentiation in normal growth media for several cell types under SW conditions, avoiding the use of growth factors and cytokines, i.e., solely by culturing cells within the SW environment. Mechanistically, it provides further insights into the balance between integrin adhesion to the dorsal substrate and the confinement imposed by the SW system.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Cellular Mechanics and Interactions · 3D Printing in Biomedical Research · Bone Tissue Engineering Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Division of Biomedical Engineering, School of Engineering, University of Glasgow. Rankine Building, Oakfield Avenue, Glasgow G12 8LT, United Kingdom

Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, University of Glasgow. Joseph Black Building, University Avenue, Glasgow G12 8QQ, United Kingdom

Escuela Técnica Superior de Ingenieros Industriales, Departamento de Termodinámica Aplicada, Universitat Politècnica de València, Camino de Vera s/n, Valencia, Valencia 46022, Spain

Instituto de Agroquímica y Tecnología de Alimentos. Consejo Superior de Investigaciones Científicas (IATA-CSIC), Departamento de Investigación: Conservación y Calidad de Alimentos,Calle Agustín Escardino 7, Paterna, Valencia 46980, Spain

Centre for Regenerative Medicine and Centre for Cardiovascular Science, University of Edinburgh. 47 Little France Crescent, Edinburgh EH16 4TJ, United Kingdom

Funding

Engineering and Physical Sciences Research Council · Consejo Superior de Investigaciones Cient?ficas · H2020 European Research Council

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