Injectable DAT-ALG Hydrogel Mitigates Senescence of Loaded DPMSCs and Boosts Healing of Perianal Fistulas in Crohn’s Disease
Source abstract
Abstract
Perianal fistulas (PAFs) are a severe complication of Crohn's disease that significantly impact patient prognosis and quality of life. While stem-cell-based strategies have been widely applied for PAF treatment, their efficacy remains limited. Our study introduces an injectable, temperature-controlled decellularized adipose tissue-alginate hydrogel loaded with dental pulp mesenchymal stem cells (DPMSCs) for in vivo fistula treatment. The experimental group demonstrated higher healing rates compared to surgical and DPMSCs groups, as evidenced by magnetic resonance imaging, multiplex immunohistochemical, and ELISA analyses. KEGG enrichment of differential genes suggested cellular senescence involvement in cell therapy efficacy, further confirmed by β-galactosidase staining and senescence markers (p21 and p53). Collectively, our research provides a novel therapy for PAFs and illuminates underlying mechanisms.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Anorectal Disease Treatments and Outcomes · Stoma care and complications · Abdominal Surgery and Complications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Gastrointestinal Surgery, Affiliated Hospital of Nantong University
Medical School of Nantong University
Research Center of Clinical Medicine
Affiliated Hospital of Nantong University
Nantong Key Laboratory of Gastrointestinal Oncology
Department of General Surgery
Guangyuan First People’s Hospital
Funding
Jiangsu Provincial Department of Education · China Postdoctoral Science Foundation · Natural Science Foundation of Jiangsu Province · Natural Science Foundation of Nantong Municipality · National Natural Science Foundation of China · Scientific and Technological Innovation and Demonstration Project of Nantong City · Foundation of Jiangsu Province Research Hospital
References (53)
Show source-provided references
1. 10.14309/ajg.000000000000232610.14309/ajg.0000000000002326 ↗
2. 10.1016/S0140-6736(16)31711-110.1016/S0140-6736(16)31711-1 ↗
3. 10.1126/sciadv.ade106710.1126/sciadv.ade1067 ↗
4. 10.3389/fimmu.2022.85995410.3389/fimmu.2022.859954 ↗
5. 10.3389/fimmu.2020.59832210.3389/fimmu.2020.598322 ↗
6. 10.1186/s13287-022-02980-310.1186/s13287-022-02980-3 ↗
7. 10.1053/j.gastro.2017.12.02010.1053/j.gastro.2017.12.020 ↗
8. 10.1007/s13577-023-00922-610.1007/s13577-023-00922-6 ↗
9. 10.1155/2020/889087310.1155/2020/8890873 ↗
10. 10.1021/acsami.4c0769410.1021/acsami.4c07694 ↗
11. 10.1016/j.jconrel.2020.11.04910.1016/j.jconrel.2020.11.049 ↗
12. 10.3389/fnmol.2022.100241910.3389/fnmol.2022.1002419 ↗
13. 10.1021/acsami.4c1255410.1021/acsami.4c12554 ↗
14. 10.3389/fcell.2021.74739810.3389/fcell.2021.747398 ↗
15. 10.1021/acsabm.4c0046610.1021/acsabm.4c00466 ↗
16. 10.1186/s12951-023-02160-210.1186/s12951-023-02160-2 ↗
17. 10.1016/j.actbio.2019.10.00110.1016/j.actbio.2019.10.001 ↗
18. 10.1038/ncomms493510.1038/ncomms4935 ↗
19. 10.1016/j.joen.2017.06.00410.1016/j.joen.2017.06.004 ↗
20. 10.1016/j.ijpharm.2016.10.04010.1016/j.ijpharm.2016.10.040 ↗
21. 10.1155/2018/897361310.1155/2018/8973613 ↗
22. 10.1002/mabi.20230035210.1002/mabi.202300352 ↗
23. 10.1016/j.biomaterials.2022.12178610.1016/j.biomaterials.2022.121786 ↗
24. 10.1016/j.bioactmat.2021.09.01410.1016/j.bioactmat.2021.09.014 ↗
25. 10.1007/s11684-021-0900-310.1007/s11684-021-0900-3 ↗
26. 10.1016/j.expneurol.2023.11450610.1016/j.expneurol.2023.114506 ↗
27. 10.1002/advs.20210352610.1002/advs.202103526 ↗
28. 10.1016/j.biomaterials.2021.12086710.1016/j.biomaterials.2021.120867 ↗
29. 10.1007/s00266-023-03608-410.1007/s00266-023-03608-4 ↗
30. 10.1007/s13770-022-00451-710.1007/s13770-022-00451-7 ↗
31. 10.1080/09205063.2015.106134910.1080/09205063.2015.1061349 ↗
32. 10.1016/j.mtbio.2022.10049810.1016/j.mtbio.2022.100498 ↗
33. 10.1016/j.ijbiomac.2021.02.08610.1016/j.ijbiomac.2021.02.086 ↗
34. 10.1088/1758-5090/ab331e10.1088/1758-5090/ab331e ↗
35. 10.1016/j.mtbio.2023.10060610.1016/j.mtbio.2023.100606 ↗
36. 10.1016/j.ijbiomac.2023.12504810.1016/j.ijbiomac.2023.125048 ↗
37. 10.1016/j.ijbiomac.2023.12712810.1016/j.ijbiomac.2023.127128 ↗
38. 10.3390/polym1321364410.3390/polym13213644 ↗
39. 10.1093/ibd/izz28810.1093/ibd/izz288 ↗
40. 10.1038/s41581-018-0023-510.1038/s41581-018-0023-5 ↗
41. 10.3389/fimmu.2022.95207110.3389/fimmu.2022.952071 ↗
42. 10.1093/stcltm/szac05410.1093/stcltm/szac054 ↗
43. 10.1016/j.lfs.2023.12218910.1016/j.lfs.2023.122189 ↗
44. 10.1053/j.gastro.2020.04.07410.1053/j.gastro.2020.04.074 ↗
45. 10.1126/scitranslmed.abq447310.1126/scitranslmed.abq4473 ↗
46. 10.1016/j.cytogfr.2023.07.00110.1016/j.cytogfr.2023.07.001 ↗
47. 10.1038/s41590-019-0415-010.1038/s41590-019-0415-0 ↗
48. 10.1038/nm.365110.1038/nm.3651 ↗
49. 10.1093/stcltm/szac00410.1093/stcltm/szac004 ↗
50. 10.1186/s11658-022-00366-010.1186/s11658-022-00366-0 ↗
51. 10.1007/s13238-021-00860-910.1007/s13238-021-00860-9 ↗
52. 10.1002/1878-0261.1361310.1002/1878-0261.13613 ↗
53. 10.1016/j.cub.2022.04.00310.1016/j.cub.2022.04.003 ↗