Cobalt Phthalocyanine-Modified Magnetic Metal–Organic Frameworks for Specific Enrichment of Phosphopeptides
Source abstract
Abstract
For mass spectrometry (MS)-based phosphoproteomics studies, sample pretreatment is an essential step for efficient identification of low-abundance phosphopeptides. Herein, a cobalt phthalocyanine-modified magnetic metal–organic framework (MOF) (Fe 3 O 4 @MIL-101-CoPc) was prepared and applied to enrich phosphopeptides before MS analysis. Fe 3 O 4 @MIL-101-CoPc exhibited an excellent magnetic response (74.98 emu g –1 ) and good hydrophilicity (7.75°), which were favorable for the enrichment. Fe 3 O 4 @MIL-101-CoPc showed good enrichment performance with high selectivity (1:1:5000), sensitivity (0.1 fmol), reusability (10 circles), and recovery (91.3%). Additionally, the Fe 3 O 4 @MIL-101-CoPc-based MS method was able to successfully detect 827 phosphopeptides from the A549 cell lysate, demonstrating a high enrichment efficiency (89.3%). This study promotes the application of postfunctionalized MOFs for phosphoproteomics analysis.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Mass Spectrometry Techniques and Applications · Advanced Proteomics Techniques and Applications · Analytical chemistry methods development
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), Inner Mongolia Minzu University, Tongliao 028000, P. R. China
Funding
National Natural Science Foundation of China
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