Metal Ion Doping of Alginate-Based Surface Coatings Induces Adipogenesis of Stem Cells
Source abstract
Abstract
Metal ions are important effectors of protein and cell functions. Here, polyelectrolyte multilayers (PEMs) made of chitosan (Chi) and alginate (Alg) were doped with different metal ions (Ca2+, Co2+, Cu2+, and Fe3+), which can form bonds with their functional groups. Ca2+ and Fe3+ ions can be deposited in PEM at higher quantities resulting in more positive ζ potentials and also higher water contact angles in the case of Fe3+. An interesting finding was that the exposure of PEM to metal ions decreases the elastic modulus of PEM. Fourier transformed infrared (FTIR) spectroscopy of multilayers provides evidence of interaction of metal ions with the carboxylic groups of Alg but not for hydroxyl and amino groups. The observed changes in wetting and surface potential are partly related to the increased adhesion and proliferation of multipotent C3H10T1/2 fibroblasts in contrast to plain nonadhesive [Chi/Alg] multilayers. Specifically, PEMs doped with Cu2+ and Fe3+ ions greatly promote cell attachment and adipogenic differentiation, which indicates that changes in not only surface properties but also the bioactivity of metal ions play an important role. In conclusion, metal ion-doped multilayer coatings made of alginate and chitosan can promote the differentiation of multipotent cells on implants without the use of other morphogens like growth factors.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Polymer Surface Interaction Studies · Bone Tissue Engineering Materials · Hydrogels: synthesis, properties, applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Pharmacy, Department Biomedical Materials, Martin Luther University Halle-Wittenberg, Heinrich-Damerow Strasse 4, 06120 Halle (Saale), Germany
Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524003, China
Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Walter-Hülse-Strasse 1, 06120 Halle (Saale), Germany
Molecular Microbiology, Institute for Biology/Microbiology, Martin-Luther-University, Halle- Wittenberg, Kurt-Mothes-Strasse 3, 06120 Halle (Saale), Germany
Institute of Physics, Martin Luther University Halle−Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Germany
Interdisciplinary Center of Materials Science, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Germany
Centre for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain
Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 46022 Valencia, Spain
Funding
Ministerio de Ciencia e Innovaci?n · European Regional Development Fund · Fraunhofer-Gesellschaft · Federal State Saxony-Anhalt · Martin-Luther-Universit?t Halle-Wittenberg
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