journal article·2021-10-11

Evaluation of Interleukin-4-Loaded Sodium Alginate–Chitosan Microspheres for Their Support of Microvascularization in Engineered Tissues

Wenhao Wang, Yuehua Liu, Zifan Liu, Shuai Li, Changxu Deng, Xiaoxiao Yang, Qian Deng, Yeye Sun, Yuxin Zhang, Zhenjiang Ma, Wentao Li, Yang Liu, Xiaojun Zhou, Tao Li, Junfeng Zhu, Jinwu Wang, Kerong Dai

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Abstract

Defects in the formation of microvascular networks, which provide oxygen and nutrients to cells, are the main reason for the engraftment failure of clinically applicable engineered tissues. Inflammatory responses and immunomodulation can promote the vascularization of the engineered tissues. We developed a capillary construct composed of a gelatin methacrylate-based cell-laden hydrogel framework complexed with interleukin-4 (IL-4)-loaded alginate-chitosan (AC) microspheres and endothelial progenitor cells (EPCs) and RAW264.7 macrophages as model cells. The AC microspheres maintained and guided the EPCs through electrostatic adhesion, facilitating the formation of microvascular networks. The IL-4-loaded microspheres promoted the polarization of the macrophages into the M2 type, leading to a reduction in pro-inflammatory factors and enhancement of the vascularization. Hematoxylin and eosin staining and immunohistochemical analysis revealed that, without IL-4 or AC microspheres, the scaffold was less effective in angiogenesis. We provide an alternative and promising approach for constructing vascularized tissues.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: 3D Printing in Biomedical Research · Angiogenesis and VEGF in Cancer · Electrospun Nanofibers in Biomedical Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

College of Medicine, Southwest Jiaotong University, Chengdu 610031, China

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, 7 Kangfu Road, Zhengzhou 450052, People’s Republic of China

School of Biological Science and Medical Engineering, BUAA, 37 Xueyuan Road, Haidian District, Beijing 100191, China

Hunan Polytechnic of Environment and Biology, Hengyang 422000, China

Department of Rehabilitation Medicine, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China

Department of Orthopaedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

Funding

Ministry of Science and Technology of the People's Republic of China · National Natural Science Foundation of China · Shanghai Science and Translational Medicine Innovation Project, Shanghai Jiao Tong University School of Medicine · Henan province medical science and technology breakthrough plan project · Fundamental Research Program, Ninth People's Hospital in affiliation with Shanghai JiaoTong University School of Medicine

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