journal article·2023-02-13

Development of a Scaffold-on-a-Chip Platform to Evaluate Cell Infiltration and Osteogenesis on the 3D-Printed Scaffold for Bone Regeneration

Jinsub Han, Sangbae Park, Jae Eun Kim, Byeongjoo Park, Yeonggeol Hong, Jae Woon Lim, Seung Jeong, Hyunmok Son, Hong Bae Kim, Hoon Seonwoo, Kyoung-Je Jang, Jong Hoon Chung

Publisher page

Abstract

Developing a scaffold for efficient and functional bone regeneration remains challenging. To accomplish this goal, a “scaffold-on-a-chip” device was developed as a platform to aid with the evaluation process. The device mimics a microenvironment experienced by a transplanted bone scaffold. The device contains a circular space at the center for scaffold insert and microfluidic channel that encloses the space. Such a design allows for monitoring of cell behavior at the blood-scaffold interphase. MC3T3-E1 cells were cultured with three different types of scaffold inserts to test its capability as an evaluation platform. Cellular behaviors, including migration, morphology, and osteogenesis with each scaffold, were analyzed through fluorescence images of live/dead assay and immunocytochemistry. Cellular behaviors, such as migration, morphology, and osteogenesis, were evaluated. The results revealed that our platform could effectively evaluate the osteoconductivity and osteoinductivity of scaffolds with various properties. In conclusion, our proposed platform is expected to replace current in vivo animal models as a highly relevant in vitro platform and can contribute to the fundamental study of bone regeneration.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: 3D Printing in Biomedical Research · Bone Tissue Engineering Materials · Additive Manufacturing and 3D Printing Technologies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biosystems Engineering, Seoul National University, Seoul 08826, Korea

Convergence Major in Global Smart Farm, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea

Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea

Department of Bio-Systems Engineering, Institute of Smart Farm, Gyeongsang National University, Jinju 52828, Korea

Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 08826, Korea

Department of Convergent Biosystems Engineering, College of Life Sciences and Natural Resources, Sunchon National University, Suncheon 57922, Korea

Interdisciplinary Program in IT-Bio Convergence System, Sunchon National University, Suncheon 57922, Korea

Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Korea

Funding

Ministry of Agriculture, Food and Rural Affairs · National Research Foundation of Korea · Seoul National University

References (45)

Show source-provided references
1. 10.1002/jps.2152810.1002/jps.21528 ↗
2. 10.1302/2046-3758.73.bjr-2017-0270.r110.1302/2046-3758.73.bjr-2017-0270.r1 ↗
3. 10.1126/scitranslmed.300489010.1126/scitranslmed.3004890 ↗
4. 10.1126/scitranslmed.aav775610.1126/scitranslmed.aav7756 ↗
5. 10.1007/s00223-018-0410-810.1007/s00223-018-0410-8 ↗
6. 10.1016/j.jot.2020.04.00710.1016/j.jot.2020.04.007 ↗
7. 10.1016/j.msec.2013.04.00210.1016/j.msec.2013.04.002 ↗
8. 10.1038/s41578-020-0204-210.1038/s41578-020-0204-2 ↗
9. 10.3390/ijms2124976510.3390/ijms21249765 ↗
10. 10.1016/j.biomaterials.2012.04.02110.1016/j.biomaterials.2012.04.021 ↗
11. 10.1002/jcp.2993510.1002/jcp.29935 ↗
12. 10.1016/j.biomaterials.2016.01.01210.1016/j.biomaterials.2016.01.012 ↗
13. 10.1016/j.msec.2014.12.03710.1016/j.msec.2014.12.037 ↗
14. 10.3877/cma.j.issn.2096-112X.2020.01.00710.3877/cma.j.issn.2096-112X.2020.01.007 ↗
15. 10.1016/j.biomaterials.2021.12120610.1016/j.biomaterials.2021.121206 ↗
16. 10.1016/j.actbio.2018.11.03910.1016/j.actbio.2018.11.039 ↗
17. 10.1021/acs.analchem.9b0498610.1021/acs.analchem.9b04986 ↗
18. 10.1002/adma.20180503310.1002/adma.201805033 ↗
19. 10.1002/admt.20180066310.1002/admt.201800663 ↗
20. 10.1021/acs.analchem.8b0500710.1021/acs.analchem.8b05007 ↗
21. 10.3390/mi1106059910.3390/mi11060599 ↗
22. 10.1039/c2ib00176d10.1039/c2ib00176d ↗
23. 10.1063/5.003652710.1063/5.0036527 ↗
24. 10.1039/c6ay01671e10.1039/c6ay01671e ↗
25. 10.1039/c5lc00685f10.1039/c5lc00685f ↗
26. Mohammadi M. S. · 2014 · 31310.1533/9780857097033.2.313 ↗
27. 10.1002/bit.2810010.1002/bit.28100 ↗
28. 10.1016/j.bone.2010.09.13810.1016/j.bone.2010.09.138 ↗
29. 10.1039/d0lc01013h10.1039/d0lc01013h ↗
30. 10.1038/s41598-019-41741-w10.1038/s41598-019-41741-w ↗
31. 10.3390/polym1302025710.3390/polym13020257 ↗
32. 10.1016/j.jbiomech.2006.09.01010.1016/j.jbiomech.2006.09.010 ↗
33. 10.1007/s10237-010-0206-x10.1007/s10237-010-0206-x ↗
34. 10.1089/ten.tea.2007.032110.1089/ten.tea.2007.0321 ↗
35. 10.1016/j.jbiomech.2008.08.01010.1016/j.jbiomech.2008.08.010 ↗
36. 10.1242/jcs.03629310.1242/jcs.036293 ↗
37. 10.1002/jbm.a.3196710.1002/jbm.a.31967 ↗
38. 10.1016/j.msec.2019.02.07310.1016/j.msec.2019.02.073 ↗
39. 10.1155/2014/31680310.1155/2014/316803 ↗
40. 10.1016/j.matdes.2018.11.01810.1016/j.matdes.2018.11.018 ↗
41. 10.1016/j.bioactmat.2019.05.00110.1016/j.bioactmat.2019.05.001 ↗
42. 10.1038/s41598-020-77103-010.1038/s41598-020-77103-0 ↗
43. 10.1016/j.msec.2020.11178710.1016/j.msec.2020.111787 ↗
44. 10.1371/journal.pone.010700110.1371/journal.pone.0107001 ↗
45. 10.1016/j.jbiosc.2015.05.01710.1016/j.jbiosc.2015.05.017 ↗