journal article·2019-11-11

pH-Triggered Silk Fibroin/Alginate Structures Fabricated in Aqueous Two-Phase System

DoYeun Park, Jie Cheng, Jong Bo Park, Sungchul Shin, Sang-Hoon Lee, Byung Hee Hong, Soo Hyun Kim, Jinho Hyun, Chaoyong Yang

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Abstract

An aqueous two-phase system (ATPS) is a water-in-water biphasic system, which is generally formed by two incompatible polymers. Recently, considerable effort has been dedicated to search for new ATPS polymer pairs to further expand ATPS's applications. In this paper, a new ATPS system based on silk fibroin (SF) and alginate is introduced. A phase diagram was established to show the critical concentrations for the formation of an SF/alginate ATPS. The present system is sensitive to pH stimulus and transformed from an ATPS into a single-phasic system as pH increases above ∼9.5. Circular dichroism, fluorescence emission spectra, hydrodynamic diameter, and ζ-potential data together indicate that the SF chains undergo a dramatic extension as pH is increased, which is the reason underlying the pH-triggered phase transition. As feasible applications of this biphasic system, compartmentalized multiplex immunoassay, controlled encapsulation and release, and hierarchical fiber fabrication were demonstrated using the SF/alginate ATPS.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Silk-based biomaterials and applications · Polymer Surface Interaction Studies · Electrospun Nanofibers in Biomedical Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

KU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea

MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

Department of Chemistry, College of Natural Science, Seoul National University, Seoul 440-746, Republic of Korea

Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea

Graduate School of Convergence Science and Technology, Seoul National University, Suwon 443-27011, Republic of Korea

Biomaterials Research Center, Korea Institute of Science and Technology, 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea

Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200127, China

Funding

Ministry of Human Resources and Social Security · KU-KIST Graduate School of Converging Science and Technology

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