DataVXACS Biomaterials Science & EngineeringPaper
journal article·2020-11-09

Human Urinal Cell Reprogramming: Synthetic 3D Peptide Hydrogels Enhance Induced Pluripotent Stem Cell Population Homogeneity

Wei Sun, Sheng Zhang, Tiancheng Zhou, Yongli Shan, Fenglin Gao, Ying Zhang, Di Zhang, Yucui Xiong, Yuanbang Mai, Ke Fan, Alan J. Davidson, Guangjin Pan, Xiao Zhang

ACS Biomaterials Science & Engineering· Volume 6· Issue 11
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Source abstract

Abstract

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs), which have promising potential applications in regenerative medicine. However, the challenges of successful applications of human iPSCs for medical purposes are the low generation efficiency, heterogeneous colonies, and exposure to the animal-derived product Matrigel. We aimed to investigate whether human urinal cells could be efficiently reprogrammed into iPSCs in three-dimensional Puramatrix (3D-PM) compared to two-dimensional Matrigel (2D-MG) and to understand how this 3D hydrogel environment affects the reprogramming process. Human urinal cells were successfully reprogrammed into iPSCs in the defined synthetic animal-free 3D-PM. Interestingly, although the colony efficiency in 3D-PM was similar to that in 2D-MG (∼0.05%), the reprogrammed colonies in 3D-PM contained an iPSC population with significantly higher homogeneity, as evidenced by the pluripotent-like morphology and expression of markers. This was further confirmed by transcriptome profile analysis in bulk cells and at the single cell level. Moreover, the homogeneity of the iPSC population in 3D-PM colonies was correlated with the downregulation of integrin β1 (ITGB1) and phosphorylated focal adhesion kinase (FAK). Collectively, 3D-PM provides an alternative approach for obtaining iPSCs with enhanced homogeneity. This work also unveiled the regulation of human somatic cell reprogramming via the extracellular microenvironment.
Source: openalex_inverted_index · Quality: baseline

Research context

Polymers

OpenAlex topics: Pluripotent Stem Cells Research · Tissue Engineering and Regenerative Medicine · 3D Printing in Biomedical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China

Guangzhou Medical University, Guangzhou 511436, China

Guangdong Provincial Key Laboratory of Biocomputing, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China

Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610051, China

Department of Molecular Medicine & Pathology, University of Auckland, Auckland 1142, New Zealand

Funding

Guangdong Science and Technology Department · China Postdoctoral Science Foundation · National Natural Science Foundation of China · Natural Science Foundation of Guangdong Province · Scientific Instrumentation Development Program of Chinese Academy of Sciences · Science and Technology Program of Guangzhou · The???Strategic???Priority???Research???Program???of???the???Chinese???Academy???of???Sciences

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