Cancer Stem Cell-Targeted Gene Delivery Mediated by Aptamer-Decorated pH-Sensitive Nanoliposomes
Source abstract
Abstract
A new pH-responsive cationic co-liposomal formulation was prepared in this study using the twin version of the amphiphile palmitoyl homocysteine, TPHC; natural zwitterionic lipid, DOPE; and cholesterol-based twin cationic lipid, C5C, at specified molar ratios. This co-liposome was further decorated with a newly designed fluorescently tagged, cholesterol-tethered EpCAM-targeting RNA aptamer for targeted gene delivery. This aptamer-guided nanoliposomal formulation, C5C/DOPE/TPHC at 8:24:1 molar ratio, could efficiently transport the genes in response to low pH of cellular endosomes selectively to the EpCAM overexpressing cancer stem cells. This particular observation was extended using siRNA against GFP to validate their transfection capabilities in response to EpCAM expression. Overall, the aptamer-guided nanoliposomal formulation was found to be an excellent transfectant for in vitro siRNA gene delivery.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques · RNA Interference and Gene Delivery · Nanopore and Nanochannel Transport Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Pediatrics, Center for Blood Oxygen Transport and Hemostasis, School of Medicine, Health Sciences Facility III, University of Maryland Baltimore, 670 W Baltimore Street, Baltimore, Maryland 21201, United States
Technical Research Centre, Indian Association for the Cultivation of Science, Kolkata 700032, India
Department of Organic Chemistry, Indian Institute of Science, Bangalore 560012, India
Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India
School of Medicine, Deakin University, Pigdons Road, Waurn Ponds, Victoria 3216, Australia
School of Applied and Interdisciplinary Sciences, Indian Association for the Cultivation of Science, Kolkata 700032, India
Funding
Department of Science and Technology, Ministry of Science and Technology
References (60)
Show source-provided references
1. 10.1038/3205810.1038/32058 ↗
2. 10.1002/jcp.2117310.1002/jcp.21173 ↗
3. 10.1038/nm0296-14410.1038/nm0296-144 ↗
4. 10.1021/cr800409e10.1021/cr800409e ↗
5. 10.1073/pnas.84.21.741310.1073/pnas.84.21.7413 ↗
6. 10.1016/j.biomaterials.2018.06.01210.1016/j.biomaterials.2018.06.012 ↗
7. 10.1016/j.jconrel.2018.08.02010.1016/j.jconrel.2018.08.020 ↗
8. 10.1021/ja204693f10.1021/ja204693f ↗
9. 10.1016/j.jconrel.2018.02.01610.1016/j.jconrel.2018.02.016 ↗
10. 10.1021/acsami.9b0948510.1021/acsami.9b09485 ↗
11. 10.1021/acsabm.0c0092410.1021/acsabm.0c00924 ↗
12. 10.1021/acsbiomaterials.9b0190410.1021/acsbiomaterials.9b01904 ↗
13. 10.1021/acsbiomaterials.0c0023110.1021/acsbiomaterials.0c00231 ↗
14. 10.1021/acsbiomaterials.0c0031610.1021/acsbiomaterials.0c00316 ↗
15. 10.1021/acsbiomaterials.0c0133610.1021/acsbiomaterials.0c01336 ↗
16. 10.1039/b900666b10.1039/b900666b ↗
17. 10.1021/acs.jpcb.6b1051110.1021/acs.jpcb.6b10511 ↗
18. 10.1021/acsnano.8b0785810.1021/acsnano.8b07858 ↗
19. 10.1021/acs.chemrev.7b0067810.1021/acs.chemrev.7b00678 ↗
20. 10.1080/17425247.2018.152083610.1080/17425247.2018.1520836 ↗
21. 10.1021/bm301066w10.1021/bm301066w ↗
22. 10.1021/acsami.6b0882310.1021/acsami.6b08823 ↗
23. 10.1039/c8nr06442c10.1039/c8nr06442c ↗
24. 10.1016/j.bmcl.2017.06.02610.1016/j.bmcl.2017.06.026 ↗
25. 10.1021/acsami.7b0706610.1021/acsami.7b07066 ↗
26. 10.1039/c7tb01080j10.1039/c7tb01080j ↗
27. 10.1021/la403694e10.1021/la403694e ↗
28. 10.1039/c5bm00479a10.1039/c5bm00479a ↗
29. 10.1016/j.jconrel.2013.06.01910.1016/j.jconrel.2013.06.019 ↗
30. Connor J. · Cancer Res. · 1986 · 46 · 3431
31. 10.1211/002235702177191310.1211/0022357021771913 ↗
32. 10.1016/j.addr.2003.10.03810.1016/j.addr.2003.10.038 ↗
33. 10.1083/jcb.101.2.58210.1083/jcb.101.2.582 ↗
34. 10.1073/pnas.81.6.171510.1073/pnas.81.6.1715 ↗
35. 10.1126/science.743402510.1126/science.7434025 ↗
36. 10.1039/c7cc03320f10.1039/c7cc03320f ↗
37. 10.1016/j.jconrel.2010.08.02710.1016/j.jconrel.2010.08.027 ↗
38. 10.1039/c4tb00701h10.1039/c4tb00701h ↗
39. 10.1016/S0169-409X(99)00062-910.1016/S0169-409X(99)00062-9 ↗
40. 10.1517/1742524100367774910.1517/17425241003677749 ↗
41. 10.1016/j.canlet.2016.03.04510.1016/j.canlet.2016.03.045 ↗
42. 10.7150/thno.2072510.7150/thno.20725 ↗
43. 10.7150/thno.2016810.7150/thno.20168 ↗
44. 10.1146/annurev-pharmtox-010716-10455810.1146/annurev-pharmtox-010716-104558 ↗
45. 10.1016/j.trac.2019.05.02710.1016/j.trac.2019.05.027 ↗
46. 10.3390/s13101362410.3390/s131013624 ↗
47. 10.1016/j.canlet.2013.07.03110.1016/j.canlet.2013.07.031 ↗
48. 10.1021/jm061125310.1021/jm0611253 ↗
49. 10.1016/S0006-3495(00)76395-810.1016/S0006-3495(00)76395-8 ↗
50. 10.1016/S0168-3659(02)00017-210.1016/S0168-3659(02)00017-2 ↗
51. 10.1016/S0168-3659(02)00167-010.1016/S0168-3659(02)00167-0 ↗
52. 10.1016/j.biomaterials.2008.12.03610.1016/j.biomaterials.2008.12.036 ↗
53. 10.1016/j.biomaterials.2013.10.06210.1016/j.biomaterials.2013.10.062 ↗
54. 10.1016/S0005-2736(98)00194-110.1016/S0005-2736(98)00194-1 ↗
55. 10.1016/j.jconrel.2013.09.01310.1016/j.jconrel.2013.09.013 ↗
56. 10.1016/S0378-5173(98)00392-510.1016/S0378-5173(98)00392-5 ↗
57. 10.1126/science.287.5454.82010.1126/science.287.5454.820 ↗
58. 10.1039/c9nr08475d10.1039/c9nr08475d ↗
59. 10.3109/10409238.2011.61459210.3109/10409238.2011.614592 ↗
60. 10.1002/jcp.104124030310.1002/jcp.1041240303 ↗