Using a linear pH-responsive zwitterionic copolymer to recover cellulases in enzymatic hydrolysate and to enhance the enzymatic hydrolysis of lignocellulose
Source abstract
Abstract
A linear pH-responsive zwitterionic copolymer P(MDB) was synthesized from functional monomers methacrylic acid, 2-(dimethylamino) ethyl methacrylate (DMA) and benzyl methacrylate (BMA) through free radical polymerization to recover cellulases and enhance the enzymatic hydrolysis. The hydrophobicity and molecular weight of the copolymer was controlled by the feed ratio of monomers and the mass ratio of initiator to monomers, respectively. UV-turbidity showed P(MDB) exhibited sensitive pH-responsiveness with dissolution–precipitation ΔpH about 1 pH unit. The precipitation pHφ of P(MDB) was 5.0–5.5 and over 90% of P(MDB) could precipitate in the vicinity of its isoelectric point. Increasing the hydrophobicity or molecular weight of P(MDB) could improve its pH sensitivity and precipitation rate. P(MDB) could enhance the enzymatic hydrolysis of dilute acid pretreated Eucalyptus by 10–25%. P(MDB) could recover the free cellulases in enzymatic hydrolysate by co-precipitation. SDS-PAGE analysis showed that P(MDB) could recover 100% β-glucosidase (β-GL) and the recovery rates of cellobiohydrolase (CBH) and endoglucanase (EG) increased as the hydrophobicity and molecular weight of P(MDB) increased. The mechanism was proposed that hydrophobic affiliation was the main binding force of P(MDB) to cellulases. Increasing the hydrophobicity and molecular weight of copolymer could increase its binding force and the number of binding sites to cellulases thus the overall cellulase recovery performance was improved.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Biofuel production and bioconversion · Advanced Cellulose Research Studies · biodegradable polymer synthesis and properties
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
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