Development of Poly(ether sulfone)/Poly(vinyl alcohol)/Magnesium-Doped Carbon Quantum Dot Scaffolds for Bone Tissue Engineering

Bone tissue engineering plays a critical role in overcoming the limitations of traditional bone grafts and implants by enhancing bone integration and regeneration. In this study, we developed a novel membrane scaffold comprising poly(ether sulfone) (PES), poly(vinyl alcohol) (PVA), and magnesium-doped carbon quantum dots (CQDs.Mg) for potential bone tissue engineering applications. Four distinct scaffold formulations (PE-CM0, PE-CM2, PE-CM3, and PE-CM4) were developed using a film applicator machine. The morphology and porosity of the scaffolds, characterized via scanning electron microscopy (SEM), revealed increased porosity with higher CQDs.Mg content. Fourier transform infrared spectroscopy (FTIR) confirmed the successful integration of functional groups from each component. Water contact angle (WCA) measurements indicated improved hydrophilicity with the addition of CQDs.Mg, which is beneficial for cell attachment and proliferation. Mechanical testing demonstrated that the scaffolds maintained adequate tensile strength and flexibility, with PE-CM3 and PE-CM4 exhibiting superior properties. Swelling assays indicated enhanced water absorption with increased CQDs.Mg content, while 14-day degradation studies showed excellent structural stability. Biocompatibility was also assessed using L929 and NIH3T3 cell lines, with cytotoxicity assays demonstrating nearly 100% cell viability across all samples. These findings suggest that the PES/PVA/CQDs.Mg scaffolds exhibit a promising combination of mechanical robustness, hydrophilicity, and biocompatibility, making them strong candidates for bone tissue engineering applications.