DataVXACS Biomaterials Science & EngineeringPaper
journal article·2024-02-12Open access

Large-Scale Preparation of Hair Follicle Germs Using a Microfluidic Device

Ellen Sugiyama, Ayaka Nanmo, Xiaolei Nie, Shu-Yung Chang, Michinao Hashimoto, Atsushi Suzuki, Tatsuto Kageyama, Junji Fukuda

ACS Biomaterials Science & Engineering· Volume 10· Issue 2
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Abstract

Hair follicle morphogenesis during embryonic development is driven by the formation of hair follicle germs (HFGs) via interactions between epithelial and mesenchymal cells. Bioengineered HFGs are potential tissue grafts for hair regenerative medicine because they can replicate interactions and hair follicle morphogenesis after transplantation. However, a mass preparation approach for HFGs is necessary for clinical applications, given that thousands of de novo hair follicles are required to improve the appearance of a single patient with alopecia. In this study, we developed a microfluidics-based approach for the large-scale preparation of HFGs. A simple flow-focusing microfluidic device allowed collagen solutions containing epithelial and mesenchymal cells to flow and generate collagen microbeads with distinct Janus structures. During the 3 days of culture, the collagen beads contracted owing to cellular traction forces, resulting in collagen- and cell-dense HFGs. The transplantation of HFGs into nude mice resulted in highly efficient de novo hair follicle regeneration. This method provides a scalable and robust tissue graft preparation approach for hair regeneration.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Hair Growth and Disorders · Dermatologic Treatments and Research · Skin and Cellular Biology Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Faculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa 240-8501, Japan

Pillar of Engineering Product Development, Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore

Digital Manufacturing and Design (DManD) Centre, Singapore University of Technology and Design, 8 Somapah Rd, Singapore 487372, Singapore

Institute of Advanced Sciences, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa 240-8501, Japan

Kanagawa Institute of Industrial Science and Technology, 3-2-1 Sakado Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan

Funding

Japan Agency for Medical Research and Development · Japan Society for the Promotion of Science · Kanagawa Institute of Industrial Science and Technology · Agency for Science, Technology and Research (A*STAR) (A*STAR-AMED joint grant) · Ministry of Education, Singapore (Academic Research Fund (AcRF) Tier 2)

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