Phosphorylated Chitosan Hydrogels Inducing Osteogenic Differentiation of Osteoblasts via JNK and p38 Signaling Pathways

Lei Liu; Yali Miao; Xuetao Shi; Huichang Gao; Yingjun Wang

doi:10.1021/acsbiomaterials.9b01374

journal article·2020-03-09

Phosphorylated Chitosan Hydrogels Inducing Osteogenic Differentiation of Osteoblasts via JNK and p38 Signaling Pathways

Lei Liu, Yali Miao, Xuetao Shi, Huichang Gao, Yingjun Wang

ACS Biomaterials Science & Engineering· Volume 6· Issue 3

Publisher page

Source abstract

Abstract

Phosphorous-containing biopolymers have been applied to expedite the regeneration of damaged bone tissue by stimulating the function of phosphorous groups in natural bones. However, the underlying mechanism of phosphorous-containing biopolymers in promoting osteogenic differentiation is unclarified. Herein, we synthesized phosphorylated chitosan hydrogels by incorporating phosphocreatine into chitosan molecular chains under mild conditions. The introduction of phosphate groups improved properties of protein adsorption and calcium deposition without affecting the morphology of hydrogels. Our results showed that phosphorylated chitosan hydrogels could not only promote alkaline phosphatase activity and mineralization but also upregulate the expression of osteogenic-related genes and proteins. Meanwhile, application of c-Jun N-terminal kinase inhibitor SP600125 and p38 mitogen-activated protein kinase inhibitor SB203580 repressed the expression of osteogenic-related markers in gene and protein levels. To the best of our knowledge, it is reported for the first time that phosphorous-containing biopolymers promote osteogenic differentiation of osteoblasts via JNK and p38 signaling pathways.

Source: openalex_inverted_index · Quality: baseline

Research context

Polymers Materials processing

OpenAlex topics: Bone Tissue Engineering Materials · Bone Metabolism and Diseases · Graphene and Nanomaterials Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

School of Material Science and Engineering, South China University of Technology, Guangzhou 510640, P. R. China

National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, P. R. China

Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou 510006, P. R. China

Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou 510006, P. R. China

Guangzhou Regenerative Medicine and Health Guangdong Laboratory, 510005 Guangzhou, P. R. China

School of Medicine, South China University of Technology, Guangzhou 510640, P. R. China

Funding

Ministry of Science and Technology of the People's Republic of China · Guangzhou Municipality · Guangdong Province · Shenzhen Municipality · National Natural Science Foundation of China · Guangzhou Regenerative Medicine and Health Guangdong Laboratory

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