DataVXACS Biomaterials Science & EngineeringPaper
journal article·2024-03-11

Aligned Nanofibers Promote Myoblast Polarization and Myogenesis through Activating Rac-Related Signaling Pathways

Xiangyu Dong, Shan Su, Qian Sun, Peng Wang, Qian Hu, Qiang Wei

ACS Biomaterials Science & Engineering· Volume 10· Issue 3
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Source abstract

Abstract

The extracellular matrix (ECM) plays a crucial role in regulating cellular behaviors and functions. However, the impact of ECM topography on muscle cell adhesion and differentiation remains poorly understood from a mechanosensing perspective. In this study, we fabricated aligned and random electrospun polycaprolactone (PCL) nanofibers to mimic the structural characteristics of ECM. Mechanism investigations revealed that the orientation of nanofibers promoted C2C12 polarization and myogenesis through Rac-related signaling pathways. Conversely, cells cultured on random fibers exhibited spreading behavior mediated by RhoA/ROCK pathways, resulting in enhanced stress fiber formation but reduced capacity for myogenic differentiation. Our findings highlight the critical role of an ECM structure in muscle regeneration and damage repair, providing novel insights into mechanosensing mechanisms underlying muscle injury diseases.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Muscle Physiology and Disorders · Cellular Mechanics and Interactions · Electrospun Nanofibers in Biomedical Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Gynecology and Obstetrics, Frontiers Science Center for Disease-related Molecular Network, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China

Department of Nephrology, Kidney Research Institute, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, P. R. China

State Key Laboratory of Polymer Materials and Engineering, College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, P. R. China

Funding

Sichuan University · Science and Technology Department of Sichuan Province · National Natural Science Foundation of China

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