Selective and Improved Photoannealing of Microporous Annealed Particle (MAP) Scaffolds

Blaise N. Pfaff; Lauren J. Pruett; Nicholas J. Cornell; Joseph de Rutte; Dino Di Carlo; Christopher B. Highley; Donald R. Griffin

doi:10.1021/acsbiomaterials.0c01580

journal article·2021-02-08Open access

Selective and Improved Photoannealing of Microporous Annealed Particle (MAP) Scaffolds

Blaise N. Pfaff, Lauren J. Pruett, Nicholas J. Cornell, Joseph de Rutte, Dino Di Carlo, Christopher B. Highley, Donald R. Griffin

ACS Biomaterials Science & Engineering· Volume 7· Issue 2

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Source abstract

Abstract

Microporous annealed particle (MAP) scaffolds consist of a slurry of hydrogel microspheres that undergo annealing to form a solid scaffold. MAP scaffolds have contained functional groups with dual abilities to participate in Michael-type addition (gelation) and radical polymerization (photoannealing). Functional groups with efficient Michael-type additions react with thiols and amines under physiological conditions, limiting usage for therapeutic delivery. We present a heterofunctional maleimide/methacrylamide 4-arm PEG macromer (MethMal) engineered for selective photopolymerization compatible with multiple polymer backbones. Rheology using two classes of photoinitiators demonstrates advantageous photopolymerization capabilities. Functional assays show benefits for therapeutic delivery and 3D printing without impacting cell viability.

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Research context

Polymers Materials processing

OpenAlex topics: 3D Printing in Biomedical Research · Photopolymerization techniques and applications · Additive Manufacturing and 3D Printing Technologies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22904, United States

Department of Bioengineering, University of California−Los Angeles, Los Angeles, California 20095, United States

Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia 22904, United States

Funding

National Institute of Diabetes and Digestive and Kidney Diseases · National Heart, Lung, and Blood Institute · Wallace H. Coulter Foundation · National Institute of General Medical Sciences

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