journal article·2020-01-13

Inhalable Dual-Targeted Hybrid Lipid Nanocore–Protein Shell Composites for Combined Delivery of Genistein and All-Trans Retinoic Acid to Lung Cancer Cells

Nayra M. Kamel, Maged W. Helmy, Elsayeda-Zeinab Abdelfattah, Sherine N. Khattab, Doaa Ragab, Magda W. Samaha, Jia-You Fang, Ahmed O. Elzoghby

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Abstract

Localized pulmonary delivery of anticancer agents to lungs has proven to be pioneering approach for lung cancer therapy. Hybrid lipid nanocore-protein shell nanoparticles (HLPNPs) coloaded with all-trans retinoic acid (ATRA) and genistein (GNS) were prepared via sequential solvent evaporation followed by nanoprecipitation of zein shell onto the lipid core. The outer protein shell of HLPNPs provided additional drug reservoir for encapsulation of ATRA/stearyl amine ion pair and enabled dual tumor-targeting with biotin and ATRA. Enhanced uptake and cytotoxic activity of HLPNPs against A549 lung cancer cells was confirmed. To improve their deep lung deposition, dual-targeted drug-loaded HLPNP nanocomposites were fabricated. The nanocomposites prepared using mannitol/HPβCD/leucine demonstrated favorable aerosolization (MMAD = 2.47 μm and FPF = 70.81%). In vivo, the inhalable nanocomposites were superior to aerosolized or i.v. nanoparticle suspension against lung carcinoma bearing mice. Overall, inhalable dual-targeted HLPNPs nanocomposites provided localized codelivery of GNS and ATRA for lung cancer therapy.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Nanoparticle-Based Drug Delivery · Inhalation and Respiratory Drug Delivery · Infant Nutrition and Health

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Cancer Nanotechnology Research Laboratory (CNRL), Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt

Department of Industrial Pharmacy, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt

Medical Research Institute, Alexandria University, Alexandria, Egypt

Department of Chemistry, Faculty of Science, Alexandria University, Alexandria 21321, Egypt

Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Taoyuan 333, Taiwan

Research Center for Industry of Human Ecology and Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Kweishan, Taoyuan 333, Taiwan

Department of Anesthesiology, Chang Gung Memorial Hospital, Kweishan, Taoyuan 333, Taiwan

Division of Engineering in Medicine, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02115, United States

Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts 02139, United States

Funding

Science and Technology Development Fund

References (69)

Show source-provided references
1. 10.1016/j.jcis.2016.07.02010.1016/j.jcis.2016.07.020 ↗
2. 10.1155/2013/86395110.1155/2013/863951 ↗
3. 10.3109/10837450.2013.85257610.3109/10837450.2013.852576 ↗
4. 10.1016/j.colsurfb.2018.05.00810.1016/j.colsurfb.2018.05.008 ↗
5. 10.3109/03639045.2014.95611110.3109/03639045.2014.956111 ↗
6. 10.1016/j.ejpb.2018.04.02310.1016/j.ejpb.2018.04.023 ↗
7. 10.1016/j.ejps.2015.10.02110.1016/j.ejps.2015.10.021 ↗
8. 10.2174/138161282366617062211125010.2174/1381612823666170622111250 ↗
9. 10.1016/j.jconrel.2010.08.02710.1016/j.jconrel.2010.08.027 ↗
10. 10.2174/138161282366617052611102910.2174/1381612823666170526111029 ↗
11. 10.1016/j.ijpharm.2016.06.11810.1016/j.ijpharm.2016.06.118 ↗
12. 10.1155/2013/25412710.1155/2013/254127 ↗
13. 10.1007/s11095-010-0329-x10.1007/s11095-010-0329-x ↗
14. 10.1016/j.ejpb.2014.03.00910.1016/j.ejpb.2014.03.009 ↗
15. 10.1016/j.jconrel.2017.11.03610.1016/j.jconrel.2017.11.036 ↗
16. 10.1016/j.ejpb.2014.02.00310.1016/j.ejpb.2014.02.003 ↗
17. 10.1007/s12013-011-9279-010.1007/s12013-011-9279-0 ↗
18. 10.1093/nar/25.15.313510.1093/nar/25.15.3135 ↗
19. 10.1186/s12951-014-0037-510.1186/s12951-014-0037-5 ↗
20. 10.1166/jbn.2012.146410.1166/jbn.2012.1464 ↗
21. 10.1007/s11095-017-2207-210.1007/s11095-017-2207-2 ↗
22. 10.3109/03639045.2014.89113010.3109/03639045.2014.891130 ↗
23. 10.2217/nnm-2017-024710.2217/nnm-2017-0247 ↗
24. 10.1016/j.ijpharm.2017.06.07710.1016/j.ijpharm.2017.06.077 ↗
25. 10.1039/C6NJ02539K10.1039/C6NJ02539K ↗
26. 10.2217/nnm-2017-030110.2217/nnm-2017-0301 ↗
27. 10.4172/2157-7439.100011010.4172/2157-7439.1000110 ↗
28. Chou T. C. · J. Biol. Chem. · 1977 · 252 · 643810.1016/S0021-9258(17)39978-7 ↗
29. 10.1016/j.powtec.2014.03.04310.1016/j.powtec.2014.03.043 ↗
30. 10.1016/j.biomaterials.2012.04.01810.1016/j.biomaterials.2012.04.018 ↗
31. 10.1007/s11051-017-3763-410.1007/s11051-017-3763-4 ↗
32. 10.1166/jbn.2011.134010.1166/jbn.2011.1340 ↗
33. 10.1016/j.colsurfb.2011.07.03610.1016/j.colsurfb.2011.07.036 ↗
34. 10.1007/s11051-012-0730-y10.1007/s11051-012-0730-y ↗
35. 10.1016/j.carbpol.2012.10.07510.1016/j.carbpol.2012.10.075 ↗
36. 10.1007/s11095-014-1300-z10.1007/s11095-014-1300-z ↗
37. 10.1016/j.ijpharm.2009.07.02510.1016/j.ijpharm.2009.07.025 ↗
38. 10.1016/j.ejpb.2014.04.01510.1016/j.ejpb.2014.04.015 ↗
39. 10.1080/1071754090275749910.1080/10717540902757499 ↗
40. 10.1186/s11671-017-1853-y10.1186/s11671-017-1853-y ↗
41. 10.1186/1556-276X-9-43410.1186/1556-276X-9-434 ↗
42. 10.1016/j.xphs.2016.03.03810.1016/j.xphs.2016.03.038 ↗
43. 10.1016/j.actbio.2015.09.03210.1016/j.actbio.2015.09.032 ↗
44. 10.3109/0190214100379015510.3109/01902141003790155 ↗
45. 10.1016/S0378-5173(02)00245-410.1016/S0378-5173(02)00245-4 ↗
46. 10.1016/j.ijpharm.2018.02.03510.1016/j.ijpharm.2018.02.035 ↗
47. 10.2174/138161282299916051115161210.2174/1381612822999160511151612 ↗
48. 10.1016/j.ejps.2013.05.01210.1016/j.ejps.2013.05.012 ↗
49. 10.1016/j.ejpb.2010.12.00210.1016/j.ejpb.2010.12.002 ↗
50. 10.1007/s11095-010-0201-z10.1007/s11095-010-0201-z ↗
51. 10.1166/jnn.2012.519210.1166/jnn.2012.5192 ↗
52. 10.1371/journal.pone.017547810.1371/journal.pone.0175478 ↗
53. 10.21577/0103-5053.2016033110.21577/0103-5053.20160331 ↗
54. 10.1016/j.ijpharm.2016.04.06710.1016/j.ijpharm.2016.04.067 ↗
55. 10.1016/j.addr.2016.01.00210.1016/j.addr.2016.01.002 ↗
56. 10.1007/s11095-011-0456-z10.1007/s11095-011-0456-z ↗
57. 10.1016/j.ejpb.2018.10.02010.1016/j.ejpb.2018.10.020 ↗
58. 10.1016/j.ejps.2016.05.02010.1016/j.ejps.2016.05.020 ↗
59. 10.1016/j.ijpharm.2016.12.00210.1016/j.ijpharm.2016.12.002 ↗
60. 10.1016/j.ijpharm.2015.02.00310.1016/j.ijpharm.2015.02.003 ↗
61. 10.1016/j.ijpharm.2011.12.04510.1016/j.ijpharm.2011.12.045 ↗
62. 10.1021/acs.molpharmaceut.5b0044310.1021/acs.molpharmaceut.5b00443 ↗
63. 10.1023/A:101104162879710.1023/A:1011041628797 ↗
64. 10.2147/IJN.S3822110.2147/IJN.S38221 ↗
65. 10.2147/IJN.S17904610.2147/IJN.S179046 ↗
66. 10.1007/s11095-014-1377-410.1007/s11095-014-1377-4 ↗
67. 10.1016/j.jconrel.2016.07.02910.1016/j.jconrel.2016.07.029 ↗
68. 10.1016/j.jconrel.2014.11.00810.1016/j.jconrel.2014.11.008 ↗
69. 10.1016/j.biomaterials.2014.04.03410.1016/j.biomaterials.2014.04.034 ↗