DataVXACS Biomaterials Science & EngineeringPaper
journal article·2021-06-14Open access

Extrusion-Based 3D Bioprinting of Gradients of Stiffness, Cell Density, and Immobilized Peptide Using Thermogelling Hydrogels

Merve Kuzucu, Grace Vera, Marco Beaumont, Sascha Fischer, Pan Wei, V. Prasad Shastri, Aurelien Forget

ACS Biomaterials Science & Engineering· Volume 7· Issue 6
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Source abstract

Abstract

To study biological processes in vitro, biomaterials-based engineering solutions to reproduce the gradients observed in tissues are necessary. We present a platform for the 3D bioprinting of functionally graded biomaterials based on carboxylated agarose, a bioink amendable by extrusion bioprinting. Using this bioink, objects with a gradient of stiffness and gradient of cell concentration were printed. Functionalization of carboxylated agarose with maleimide moieties that react in minutes with a cysteine-terminated cell-adhesion peptide allowed us to print objects with a gradient of an immobilized peptide. This approach paves the way toward the development of tissue mimics with gradients.
Source: europepmc · Quality: candidate unverified · Source record ↗

Research context

PolymersBiomaterials

OpenAlex topics: 3D Printing in Biomedical Research · Innovative Microfluidic and Catalytic Techniques Innovation · Cellular Mechanics and Interactions

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute for Macromolecular Chemistry, University of Freiburg, Stefan-Meier-Str. 31, 79104 Freiburg, Germany

School of Chemistry and Physics, Queensland University of Technology, 2 George St, Brisbane City, Queensland 4000, Australia

Institute of Chemistry of Renewable Resources, University of Natural Resources and Life Sciences (BOKU), Konrad-Lorenz-Straße 24 3430 Tulln, Austria

BIOSS, Centre for Cell Signalling Studies, Schänzlestr. 18, 79104 Freiburg, Germany

Funding

Austrian Science Fund · Albert-Ludwigs-Universit?t Freiburg · Secretariat of Higher Education, Science, Technology and Innovation

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