Interfacial assembly of mesoporous nanopyramids as ultrasensitive cellular interfaces featuring efficient direct electrochemistry
Source abstract
Abstract
A general method for assembling patterned interfaces of uniform, flexible mesoporous iron oxide nanopyramid islands (NPIs) is presented. The three-dimensional (3D) mesoporous iron oxide–NPI interfaces possess a unique mesostructure that features a large surface area (~158 m2 g−1), a large pore size (~18 nm) and excellent flexibility (can be folded 100 times). Furthermore, the 3D mesoporous Au–NPI interfaces allow efficient immobilization of cytochrome c (Cyt c; more than 165-fold increase) and a significant enhancement of localized surface plasmon resonance (~26-fold at 625 nm) compared with that of two-dimensional (2D) planar iron oxide films without nanopores. More importantly, the ultrasensitive integrated interfaces demonstrate over 1000-fold enhancement of the photocurrent variation on the 3D mesostructures based on the switchable direct electrochemistry of Cyt c. The strategy of interfacial assembly offers new possibilities for the chemical design of patterned mesoporous semiconductors with high flexibility and tailored photocatalytic characteristics. This investigation provides a novel paradigm for an unconventional 3D porous biointerface that can be used for sub-nanomolar level recognition of biomolecules (~0.2 nM for H2O2) and suggests the new concept of large-surface-area 3D mesostructure–protein interfaces as a step toward using direct electrochemistry for biomedical applications. Flexible lab-on-a-chip devices containing iron oxide nanopyramids may kick start new approaches to sub-nanomolar detection of biomolecules. A team of Chinese and Australian researchers led by Dongyuan Zhao from Fudan University used lithographic techniques to pattern surfaces with pointy iron oxide nanopyramids and gold nanoparticles and then loaded the protein cytochrome c (Cyt c). The three-dimensional, mesoporous cavities inside the nanopyramids boosted both cell attachment onto the device and the surface plasmon signals used for biosensing. When the team exposed the biointerface to solar light to initiate the Cyt c redox cycle, they saw a 1,000-fold enhancement in the photoelectrochemical current over typical interfaces. The magnitude of such solar-driven currents makes the device extremely sensitive — the researchers foresee direct monitoring of electrochemical transfer between proteins for real-time disease diagnostics. A general method for assembling patterned interfaces of uniform, flexible mesoporous iron oxide nanopyramid islands is presented. The 3D porous interfaces possess a unique mesostructure that features a large surface area, a large pore size and excellent flexibility. Furthermore, the 3D porous Au–NPI interfaces allow efficient immobilization of cytochrome c and a significant enhancement of localized surface plasmon resonance. More importantly, the ultrasensitive integrated interfaces demonstrate over 1000-fold enhancement of the photocurrent variation on the 3D mesostructures based on the switchable direct electrochemistry cytochrome c. The strategy of interfacial assembly offers new possibilities for the chemical design of patterned mesoporous semiconductors with high flexibility and tailored photocatalytic characteristics.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques · Iron oxide chemistry and applications · Anodic Oxide Films and Nanostructures
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (55)
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