Delivery of molecules into cells using carbon nanoparticles activated by femtosecond laser pulses
Source abstract
Abstract
A major barrier to drug and gene delivery is crossing the cell's plasma membrane. Physical forces applied to cells via electroporation, ultrasound and laser irradiation generate nanoscale holes in the plasma membrane for direct delivery of drugs into the cytoplasm. Inspired by previous work showing that laser excitation of carbon nanoparticles can drive the carbon-steam reaction to generate highly controlled shock waves, we show that carbon black nanoparticles activated by femtosecond laser pulses can facilitate the delivery of small molecules, proteins and DNA into two types of cells. Our initial results suggest that interaction between the laser energy and carbon black nanoparticles may generate photoacoustic forces by chemical reaction to create transient holes in the membrane for intracellular delivery.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Microbial Inactivation Methods · Spectroscopy Techniques in Biomedical and Chemical Research · Carbon Nanotubes in Composites
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (31)
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1. Bodles-Brakhop, A. M., Heller, R. & Draghia-Akli, R. Electroporation for the delivery of DNA-based vaccines and immunotherapeutics: current clinical developments. Mol. Ther. 17, 585–592 (2009).10.1038/mt.2009.5 ↗
2. Mitragotri, S. Healing sound: the use of ultrasound in drug delivery and other therapeutic applications. Nature Rev. Drug Discov. 4, 255–260 (2005).10.1038/nrd1662 ↗
3. Tao, W., Wilkinson, J., Stanbridge, E. J. & Berns, M. W. Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane. Proc. Natl Acad. Sci. USA 84, 4180–4184 (1987).10.1073/pnas.84.12.4180 ↗
4. Tirlapur, U. K. & Konig, K. Targeted transfection by femtosecond laser. Nature 418, 290–291 (2002).10.1038/418290a ↗
5. Kodama, T., Doukas, A. G. & Hamblin, M. R. Shock wave-mediated molecular delivery into cells. Biochim. Biophys. Acta 1542, 186–194 (2002).10.1016/S0167-4889(01)00177-X ↗
6. Esenaliev, R. O., Larina, I. V., Larin, K. V., Motamedi, M. & Evers, B. M. Mechanism of laser-induced drug delivery in tumors. Proc. SPIE 3914, 188–196 (2000).10.1117/12.388045 ↗
7. Löwen, H. & Madden, P. A. A microscopic mechanism for shock-wave generation in pulsed-laser-heated colloidal suspensions. J. Chem. Phys. 97, 8760–8766 (1992).10.1063/1.463345 ↗
8. Chen, H. & Diebold, G. Chemical generation of acoustic waves: a giant photoacoustic effect. Science 270, 963–966 (1995).10.1126/science.270.5238.963 ↗
9. Chen, H., McGrath, T. & Diebold, G. J. Laser chemistry in suspensions: new products and unique reaction conditions for the carbon-steam reaction. Angew. Chem. Int. Ed. Engl. 36, 163–166 (1997).10.1002/anie.199701631 ↗
10. McGrath, T. E., Diebold, G. J., Bartels, D. M. & Crowell, R. A. Laser-initiated chemical reactions in carbon suspensions. J. Phys. Chem. A 106, 10072–10078 (2002).10.1021/jp0208135 ↗
11. Lu, S., Wang, S. & Grimes-Serrano, J. M. Current progress of DNA vaccine studies in humans. Expert Rev. Vaccines 7, 175–191 (2008).10.1586/14760584.7.2.175 ↗
12. Whitehead, K. A., Langer, R. & Anderson, D. G. Knocking down barriers: advances in siRNA delivery. Nature Rev. Drug Discov. 8, 129–138 (2009).10.1038/nrd2742 ↗
13. Desnick, R. J. Enzyme replacement and enhancement therapies for lysosomal diseases. J. Inherit. Metab. Dis. 27, 385–410 (2004).10.1023/B:BOLI.0000031101.12838.c6 ↗
14. Nobili, S., Landini, I., Giglioni, B. & Mini, E. Pharmacological strategies for overcoming multidrug resistance. Curr. Drug Targets 7, 861–879 (2006).10.2174/138945006777709593 ↗
15. Young, L. S., Searle, P. F., Onion, D. & Mautner, V. Viral gene therapy strategies: from basic science to clinical application. J. Pathol. 208, 299–318 (2006).10.1002/path.1896 ↗
16. Lavigne, M. D. & Gorecki, D. C. Emerging vectors and targeting methods for nonviral gene therapy. Expert Opin. Emerg. Drugs 11, 541–557 (2006).10.1517/14728214.11.3.541 ↗
17. Mehier-Humbert, S. & Guy, R. H. Physical methods for gene transfer: improving the kinetics of gene delivery into cells. Adv. Drug Deliv. Rev. 57, 733–753 (2005).10.1016/j.addr.2004.12.007 ↗
18. Esenaliev, R. O. Application of light and ultrasound for medical diagnostics and treatment. Proc. SPIE 4707, 158–164 (2002).10.1117/12.475583 ↗
19. ter Haar, G. Therapeutic applications of ultrasound. Prog. Biophys. Mol. Biol. 93, 111–129 (2007).10.1016/j.pbiomolbio.2006.07.005 ↗
20. Hernot, S. & Klibanov, A. L. Microbubbles in ultrasound-triggered drug and gene delivery. Adv. Drug Deliv. Rev. 60, 1153–1166 (2008).10.1016/j.addr.2008.03.005 ↗
21. Hynynen, K. Ultrasound for drug and gene delivery to the brain. Adv. Drug Deliv. Rev. 60, 1209–1217 (2008).10.1016/j.addr.2008.03.010 ↗
22. Lukacs, G. L. et al. Size-dependent DNA mobility in cytoplasm and nucleus. J. Biol. Chem. 275, 1625–1629 (2000).10.1074/jbc.275.3.1625 ↗
23. Sun, X. et al. Investigation of an optical limiting mechanism in multiwalled carbon manotubes. Appl. Opt. 39, 1998–2001 (2000).10.1364/AO.39.001998 ↗
24. Link, S., Burda, C., Nikoobakht, B. & El-Sayed, M. A. Laser-induced shape changes of colloidal gold nanorods using femtosecond and nanosecond laser pulses. J. Phys. Chem. B 104, 6152–6163 (2000).10.1021/jp000679t ↗
25. Fayer, M. Picosecond holographic grating generation of ultrasonic waves. IEEE J. Quant. Electron. 22, 1437–1452 (1986).10.1109/JQE.1986.1073140 ↗
26. Brukh, R. & Mitra, S. Kinetics of carbon nanotube oxidation. J. Mater. Chem. 17, 619–623 (2007).10.1039/B609218G ↗
27. Zhao, N., He, C., Jiang, Z., Li, J. & Li, Y. Physical activation and characterization of multi-walled carbon nanotubes catalytically synthesized from methane. Mater. Lett. 61, 681–685 (2007).10.1016/j.matlet.2006.05.041 ↗
28. Huang, X., El-Sayed, I. H., Qian, W. & El-Sayed, M. A. Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. J. Am. Chem. Soc. 128, 2115–2120 (2006).10.1021/ja057254a ↗
29. Zarnitsyn, V. G. & Prausnitz, M. R. Physical parameters influencing optimization of ultrasound-mediated DNA transfection. Ultrasound Med. Biol. 30, 527–538 (2004).10.1016/j.ultrasmedbio.2004.01.008 ↗
30. Guzman, H. R., Nguyen, D. X., Khan, S. & Prausnitz, M. R. Ultrasound-mediated disruption of cell membranes I: quantification of molecular uptake and cell viability. J. Acoust. Soc. Am. 110, 588–596 (2001).10.1121/1.1376131 ↗
31. Schlicher, R. K. et al. Mechanism of intracellular delivery by acoustic cavitation. Ultrasound Med. Biol. 32, 915–924 (2006).10.1016/j.ultrasmedbio.2006.02.1416 ↗