journal article·2026-06-24

Label-Free and In Situ Glycophenotyping Using a Glycocalyx-Mimetic Electrochemical Transducer

Thiago Coimbra Pimenta, Alexandre Xavier Mendes, Ronil J. Rath, Luiza Aguiar do Nascimento, Vatsala Pithaih, Henry F. F. Bellette, Eliza Grills, Andreas Behren, Dênio E. Pires Souto, George Wren Greene, Simon E. Moulton, Jessica Da Gama Duarte, Saimon M. Silva

ACS Applied Materials & Interfaces· Volume 18· Issue 24
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Abstract

-glycosylated mucin domains present tumor-associated truncated O-glycans that serve as linkers to the lectins, our recognition elements. Embedding six lectins within lubricin enables orthogonal recognition of Gal-GalNAc-terminated oligosaccharides. In an electrochemical culture-plate format, the resulting sensor supports real-time, in situ glycophenotyping of melanoma cells, achieving quantitative agreement to a conventional, end point, lectin microarray. This glycocalyx-mimetic transducer thus enables the rapid, label-free screening of lectin-glycan interactions and the profiling of diagnostically relevant, tumor-associated glycans.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Glycosylation and Glycoproteins Research · Galectins and Cancer Biology · Carbohydrate Chemistry and Synthesis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biochemistry and Chemistry, School of Agriculture, Biomedicine and Environment, La Trobe University, Melbourne, Victoria 3086, Australia

The Biomedical and Environmental Sensor Technology (BEST) Research Centre, La Trobe Institute of Molecular Science (LIMS), La Trobe University, Melbourne, Victoria 3086, Australia

ARC Research Hub for Molecular Biosensors at Point-of-Use (MOBIUS), La Trobe University, Melbourne, Victoria 3086, Australia

School of Engineering, Swinburne University of Technology, Melbourne, Victoria 3122, Australia

Aikenhead Centre for Medical Discovery, St Vincent’s Hospital, Melbourne, Victoria 3065, Australia

Department of Cancer Medicine, School of Translational Medicine, Monash University, Melbourne, Victoria 3004, Australia

Olivia Newton-John Cancer Research Institute, and La Trobe School of Cancer Medicine, La Trobe University, Heidelberg, Victoria 3084, Australia

Laboratorio de Espectrometria

Sensores e Biossensores─Department of ChemistryFederal University of Paraná (UFPR)

Funding

Australian Research Council (ARC)

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