journal article·2026-06-17

Multifunctional Inverse Opal Nerve Guidance Conduits Loaded with Mesenchymal Stem Cells for Peripheral Nerve Repair

Lei Ren, Xin Gao, Hui Zhang, Junbo Zhang, Hong Cheng, Jing Li, Yusong Wang, Lei Tian, Yangnan Hu, Jingying Ye, Hao Wu, Huan Wang, Renjie Chai

ACS Applied Materials & Interfaces· Volume 18· Issue 23
Publisher page

Abstract

Nerve guidance conduits (NGCs) hold considerable value in the field of nerve regeneration, yet current approaches display constraints regarding the diversity of loaded factors and their short sustained-release duration. Herein, a type of multifunctional inverse opal NGC integrating bone marrow mesenchymal stem cells (BMSCs) is presented for peripheral nerve repair. The generation of inverse opal scaffolds leverages the inversion of a monodisperse emulsion droplet template crafted by microfluidic technique. Ascribed to the biocompatibility and the cell-concentrating properties of the inverse opal scaffold, coupled with the inherent capabilities of BMSCs, such an NGC enables the secretion of nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor, promoting the migration of rat Schwann cells and differentiation of pheochromocytoma 12 cells. Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) further revealed that three-dimensional (3D) BMSC spheroid culture in the inverse opal scaffold significantly upregulated bFGF, PDGF, and VEGF mRNA expression relative to two-dimensional (2D) culture. Further in vivo experimentation confirms the promising efficacy of NGCs loaded with BMSCs in repairing 10 mm sciatic nerve defects. These results underscore the substantial potential of this innovative design for peripheral nerve regeneration.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Nerve injury and regeneration · Silk-based biomaterials and applications · 3D Printing in Biomedical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High Tech Key Laboratory for Bio-Medical Research

Southeast University

Co-Innovation Center of Neuroregeneration

Nantong University

Department of Otolaryngology, Head and Neck Surgery, Sleep Medicine Center, Beijing Tsinghua Changgung Hospital (Beijing Key Laboratory for Sleep Breathing Disorder), School of Clinical Medicine

Tsinghua University

Department of Neurosurgery

Xuanwu Hospital, Capital Medical University

The Eighth Affiliated Hospital

Sun Yat-Sen University

Department of Neurology, Aerospace Center Hospital, School of Life Science

Beijing Institute of Technology

Funding

National Postdoctoral Program for Innovative Talents · Shenzhen Municipal Fundamental Research Program · Basic and Applied Basic Research Foundation of Guangdong Province · China Postdoctoral Science Foundation · Shenzhen Medical Research Fund · Beijing Municipal Natural Science Foundation · Jiangsu Funding Program for Excellent Postdoctoral Talent · National Basic Research Program of China (973 Program) · National Natural Science Foundation of China (NSFC) · Open Project Fund of Guangdong Academy of Medical Sciences

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