Comment on “Monoclonal Antibody Discovery Based on Precise Selection of Single Transgenic Hybridomas with an On-Cell-Surface and Antigen-Specific Anchor”
Source abstract
Abstract
In the original paper, Li and co-workers [ ACS Appl. Mater. Interfaces 2022, 14, 17128–17141] described their approach to select specific hybridoma cells from a polyclonal hybridoma pool by using a cell surface anchor to catch the secreted antibody. The antigen-specific detection was performed with streptavidin-labeled antigen and a PE-labeled anti-F(ab′) 2 antibody. The present comment offers a clearer description of the selection system originally published by Listek et al. in 2020 and provides further information about the importance of controls and recent adaptations made by our lab.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Monoclonal and Polyclonal Antibodies Research · Viral Infectious Diseases and Gene Expression in Insects · Protein purification and stability
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Immunotechnology Group, Institute of Biochemistry and Biology, University of Potsdam, 14469 Potsdam, Germany
new/era/mabs GmbH, August-Bebel-Str. 89, 14482 Potsdam, Germany
Berlin-Brandenburg Center for Regenerative Therapies (BCRT), 13353 Berlin, Germany
Helmholtz-Zentrum Geesthacht, Institute of Active Polymers, 14513 Teltow, Germany
Funding
Bundesministerium für Bildung und Forschung · European Commission
References (9)
Show source-provided references
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