Construction of Coumarin-Based Bioorthogonal Macromolecular Probes for Photoactivation

Photoactivatable fluorescence imaging is one of the most valuable methods for visualizing protein localization, trafficking, and interactions. Here, we designed four bioorthogonal fluorescent probes K1 – K4 by installing photoactive cages and HaloTag ligands onto the different positions of the coumarin fluorophore. Although K1 – K4 all exhibited rapid photostimulated responses in aqueous solution, only K3 was found to have an obvious aggregation-induced emission (AIE). Next, macromolecular fluorescent probes K n =1/2/3/4 _ POIs were obtained by covalently attaching K1 – K4 to HaloTag-fused proteins of interest (POIs). K n =3/4 _ POIs exhibited a higher fluorescence increase than that of K n =1/2 _ POIs upon photoactivation in both liquid and solid phases. Moreover, K3 _ GFP _ Halo and K4 _ GFP _ Halo presented the fluorescence resonance energy transfer (FRET) from photocleaved K3 and K4 to GFP in the protein complex. We further examined the fluorescence labeling ability of K1 – K4 to intracellular IRE1_Halo protein and found that K3 and K4 containing the HaloTag ligand on the C4 position of coumarin could be retained in cells for long-term tracking of the IRE1_Halo protein. Hence, we established a platform of novel bioorthogonal fluorescent probes conjugating onto Halo-tagged POIs for rapid photoactivation in vitro and in cells.