journal article·2023-11-08

Spatiotemporal Controlled Formation of Synthetic Nanoreactors in Single Living Cells

Jian Lv, Xiao-Yuan Wang, Xin-Yue Zhou, Xue Wu, Shuai Chang, Bin-Bin Chen, Zhi-Qian Guo, Da-Wei Li, Ruo-Can Qian

ACS Applied Materials & Interfaces· Volume 15· Issue 44
Publisher page

Abstract

Cellular compartments provide confined environments for spatiotemporal control of biological processes and enzymatic reactions. To mimic such compartmentalization of eukaryotic cells, we report an efficient and general platform to precisely control the formation of artificial nanoreactors in single living cells. We introduce an electroosmotic controlled strategy for the synthesis of ZIF-8 at the nanoscale liquid-liquid interface around the tip of a nanopipet, whereby the formed ZIF-8 nanoparticles are driven into a single living cell by the electroosmotic flow. The porous ZIF-8 nanoparticles, as synthetic nanoreactors, are not only able to harvest fluorescent molecules from peripheral cytoplasm but also perform the subsequent photocatalytic degradation, mimicking compartmentalized chemical reactions in eukaryotic cells. Our strategy provides a useful tool for spatiotemporal controlled synthesis of artificial nanoreactors with on-demand functions in single living cells with versatile applications in chemical biology.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Nanopore and Nanochannel Transport Studies · Supramolecular Self-Assembly in Materials · Lipid Membrane Structure and Behavior

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China

Shenzhen Institute of Aggregate Science and Technology, School of Science and Engineering, The Chinese University of Hong Kong, Shenzhen, 2001 Longxiang Boulevard, Longgang District, Shenzhen, Guangdong 518172, China

Funding

Project 211 · China Postdoctoral Science Foundation · Science and Technology Commission of Shanghai Municipality · Fundamental Research Funds for the Central Universities · National Natural Science Foundation of China

References (35)

Show source-provided references
1. 10.1038/nrmicro.2018.1010.1038/nrmicro.2018.10 ↗
2. 10.1126/science.122372810.1126/science.1223728 ↗
3. 10.1016/j.tcb.2018.02.00410.1016/j.tcb.2018.02.004 ↗
4. 10.1126/science.aaf438210.1126/science.aaf4382 ↗
5. 10.1002/ange.20221876610.1002/ange.202218766 ↗
6. 10.1021/jacs.0c1249410.1021/jacs.0c12494 ↗
7. 10.1002/smll.20220229410.1002/smll.202202294 ↗
8. 10.1038/s41467-018-03560-x10.1038/s41467-018-03560-x ↗
9. 10.1021/acs.nanolett.6b0343010.1021/acs.nanolett.6b03430 ↗
10. 10.1002/adfm.20200294910.1002/adfm.202002949 ↗
11. 10.1038/s41589-020-0579-910.1038/s41589-020-0579-9 ↗
12. 10.1002/ange.20221696610.1002/ange.202216966 ↗
13. 10.1002/anie.20210625910.1002/anie.202106259 ↗
14. 10.1021/ja066019510.1021/ja0660195 ↗
15. 10.1073/pnas.060243910310.1073/pnas.0602439103 ↗
16. 10.1021/acs.nanolett.0c0268310.1021/acs.nanolett.0c02683 ↗
17. 10.1021/jacs.0c0414410.1021/jacs.0c04144 ↗
18. 10.1002/anie.20170973810.1002/anie.201709738 ↗
19. 10.1016/j.ijhydene.2021.10.18010.1016/j.ijhydene.2021.10.180 ↗
20. 10.1126/science.123044410.1126/science.1230444 ↗
21. 10.1038/s41565-020-00788-x10.1038/s41565-020-00788-x ↗
22. 10.1021/jacs.7b0122910.1021/jacs.7b01229 ↗
23. 10.1021/acssensors.1c0046310.1021/acssensors.1c00463 ↗
24. 10.1039/C6CC08125H10.1039/C6CC08125H ↗
25. 10.1021/acs.analchem.1c0331310.1021/acs.analchem.1c03313 ↗
26. 10.1021/acs.analchem.9b0418510.1021/acs.analchem.9b04185 ↗
27. 10.1039/c0cc05002d10.1039/c0cc05002d ↗
28. 10.1002/anie.20200714610.1002/anie.202007146 ↗
29. 10.1021/acs.analchem.8b0415910.1021/acs.analchem.8b04159 ↗
30. 10.1021/ac202434c10.1021/ac202434c ↗
31. 10.1021/ac402176910.1021/ac4021769 ↗
32. 10.1021/acs.analchem.1c0237110.1021/acs.analchem.1c02371 ↗
33. 10.1021/acs.langmuir.9b0269210.1021/acs.langmuir.9b02692 ↗
34. 10.1002/anie.20160829910.1002/anie.201608299 ↗
35. 10.1016/j.cell.2021.09.00710.1016/j.cell.2021.09.007 ↗