Engineered Escherichia coli Consortia Function in a Programmable Pattern for Multiple Enzymatic Biosynthesis

Wenxue Zhang; Hao Dong; Xiaoli Wang; Liting Zhang; Chao Chen; Ping Wang

doi:10.1021/acsami.3c09123

journal article·2023-10-04

Engineered Escherichia coli Consortia Function in a Programmable Pattern for Multiple Enzymatic Biosynthesis

Wenxue Zhang, Hao Dong, Xiaoli Wang, Liting Zhang, Chao Chen, Ping Wang

ACS Applied Materials & Interfaces· Volume 15· Issue 39

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Source abstract

Abstract

Coordinating microbial consortia to realize complex synthetic pathways is an area of great interest in the rapidly growing field of biomanufacturing. This work presents a programmable method for assembling living cells based on the surface display of affinity groups, enabling whole-cell catalysis with optimized catalytic efficiency through the rational arrangement of cell assemblies and enzymes. In the context of d-phenyllactic acid (d-PLA) synthesis, four enzymes were rationally arranged considering substrate channeling and protein expression levels. The production efficiencies of d-PLA catalyzed by engineered microbial consortia were 1.31- and 2.55-fold higher than those of biofilm and whole-cell catalysts, respectively. Notably, substrate channeling was identified between the coimmobilized rate-limiting enzymes, resulting in a 3.67-fold improvement in catalytic efficiency compared with hybrid catalysts (free enzymes coupled with whole-cell catalysts). The highest yield of d-PLA catalyzed by microbial consortia was 102.85 ± 3.39 mM with 140 mM benzaldehyde as the substrate. This study proposes a novel approach to cell enzyme assembly for coordinating microbial consortia in multiple enzymatic biosynthesis processes.

Source: openalex_inverted_index · Quality: baseline

Research context

Catalysis & surfaces

OpenAlex topics: Enzyme Catalysis and Immobilization · Microbial Metabolic Engineering and Bioproduction · Innovative Microfluidic and Catalytic Techniques Innovation

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China

College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province, Zhejiang University of Science and Technology, Hangzhou 310023, China

Institute for Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China

Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, Minnesota 55108, United States

Funding

Natural Science Foundation of Shanghai Municipality · China Postdoctoral Science Foundation · Fundamental Research Funds for the Central Universities · National Natural Science Foundation of China · Shanghai Rising-Star Program · Basic Public Welfare Project of Zhejiang Province · Qingdao Postdoctoral Applied Research Project

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