DataVXACS Applied Materials & InterfacesPaper
journal article·2022-10-26Open access

Hierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion

Marc Martínez-Miguel, Miquel Castellote-Borrell, Mariana Köber, Adriana R. Kyvik, Judit Tomsen-Melero, Guillem Vargas-Nadal, Jose Muñoz, Daniel Pulido, Edgar Cristóbal-Lecina, Solène Passemard, Miriam Royo, Marta Mas-Torrent, Jaume Veciana, Marina I. Giannotti, Judith Guasch, Nora Ventosa, Imma Ratera

ACS Applied Materials & Interfaces· Volume 14· Issue 42
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Source abstract

Abstract

The synthesis and study of the tripeptide Arg-Gly-Asp (RGD), the binding site of different extracellular matrix proteins, e.g., fibronectin and vitronectin, has allowed the production of a wide range of cell adhesive surfaces. Although the surface density and spacing of the RGD peptide at the nanoscale have already shown a significant influence on cell adhesion, the impact of its hierarchical nanostructure is still rather unexplored. Accordingly, a versatile colloidal system named quatsomes, based on fluid nanovesicles formed by the self-assembling of cholesterol and surfactant molecules, has been devised as a novel template to achieve hierarchical nanostructures of the RGD peptide. To this end, RGD was anchored on the vesicle's fluid membrane of quatsomes, and the RGD-functionalized nanovesicles were covalently anchored to planar gold surfaces, forming a state of quasi-suspension, through a long poly(ethylene glycol) (PEG) chain with a thiol termination. An underlying self-assembled monolayer (SAM) of a shorter PEG was introduced for vesicle stabilization and to avoid unspecific cell adhesion. In comparison with substrates featuring a homogeneous distribution of RGD peptides, the resulting hierarchical nanoarchitectonic dramatically enhanced cell adhesion, despite lower overall RGD molecules on the surface. The new versatile platform was thoroughly characterized using a multitechnique approach, proving its enhanced performance. These findings open new methods for the hierarchical immobilization of biomolecules on surfaces using quatsomes as a robust and novel tissue engineering strategy.
Source: openalex_inverted_index · Quality: baseline

Research context

NanomaterialsBiomaterials

OpenAlex topics: Cell Adhesion Molecules Research · Lipid Membrane Structure and Behavior · Monoclonal and Polyclonal Antibodies Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain

Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain

Unidad de Péptidos, UB, Unidad asociada al CSIC por el IQAC, Barcelona 08028, Spain

Institut de Química Avançada de Catalunya (IQAC−CSIC), Barcelona 08034, Spain

Nanoprobes and Nanoswitches group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Barcelona 08028, Spain

Departament de Ciència dels Materials i Química Física, Universitat de Barcelona, Barcelona 08028, Spain

Dynamic Biomimetics for Cancer Immunotherapy, Max Planck Partner Group, ICMAB-CSIC, Campus UAB, Bellaterra 08193, Spain

Funding

Centro de Investigaci?n Biom?dica en Red en Bioingenier?a, Biomateriales y Nanomedicina · European Regional Development Fund · H2020 Leadership in Enabling and Industrial Technologies · Ag?ncia de Gesti? d?Ajuts Universitaris i de Recerca · Generalitat de Catalunya · Universitat Aut?noma de Barcelona · Fundaci? la Marat? de TV3 · Ministerio de Ciencia, Innovaci?n y Universidades · Ministerio de Econom?a y Competitividad · H2020 Marie Sklodowska-Curie Actions · European Cooperation in Science and Technology · Max-Planck-Gesellschaft · Instituto de Salud Carlos III · European Social Fund

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