Surface-Displayed Antibodies as a Tool for Simultaneously Controlling the Arrangement and Morphology of Multiple Cell Types with Microscale Precision

Cell-cell interactions are considered to play critical roles in the development and physiology of most tissues. However, it is not straightforward to analyze cell-cell interactions with conventional cell culture in which cells are randomly distributed. To overcome this limitation, we employed here an antibody display to sort different cell types onto separate regions on a single substrate with microscale precision, taking advantage of the specific recognition of cell surface markers by surface-displayed antibodies. The results obtained with two sets of cell combinations, T cell/myelomonocytoid cell and neuron/astrocyte, demonstrate that antibody displays are feasible to establish a site-addressable coculture.