DataVXACS Applied Materials & InterfacesPaper
journal article·2019-12-04

High-Throughput Identification and Screening of Single Microbial Cells by Nanobowl Array

Xiuyan Li, Hongqing Feng, Zhe Li, Yue Shi, Jingjing Tian, Chaochao Zhao, Min Yu, Zhuo Liu, Hu Li, Bojing Shi, Qian Wang, Luhai Li, Dongshu Wang, Li Zhu, Ruping Liu, Zhou Li

ACS Applied Materials & Interfaces· Volume 11· Issue 48
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Source abstract

Abstract

High-throughput screening and fast identification of single bacterial cells are crucial for clinical diagnosis, bioengineering, and fermentation engineering. Although single-cell technologies have been developed extensively in recent years, the single-cell technologies for bacteria still need further exploration. In this study, we demonstrate an identification and screening technology for single bacterial cells based on a large-scale nanobowl array, which is well-ordered and size-adjustable for use with different kinds of bacteria. When the culture medium with monodispersed bacteria was placed on the nanobowl array, it successfully enabled loading of single bacterium into a single nanobowl. Because of the limitative size and depth of the nanobowls, mixture of different bacteria species could be screened according to their sizes. In addition, with the help of a low electrical current, the bacteria can be further screened according to their intrinsic surface charges. If combined with micromanipulation technology, high-throughput single bacterial selection can be achieved in future.
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Research context

Nanomaterials

OpenAlex topics: Innovative Microfluidic and Catalytic Techniques Innovation · Microfluidic and Bio-sensing Technologies · 3D Printing in Biomedical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Beijing Institute of Graphic Communication, Beijing 102600, P. R. China

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, P. R. China

School of Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100049, P. R. China

School of Stomatology and Medicine, Foshan University, Foshan 528000, P. R. China

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, P. R. China

Center on Nanoenergy Research, School of Physical Science and Technology, Guangxi University, Nanning 530004, P. R. China

Funding

Ministry of Science and Technology of the People's Republic of China · Natural Science Foundation of Beijing Municipality · Beijing Municipal Science and Technology Commission · Beijing Institute of Graphic Communication · National Natural Science Foundation of China · Guangdong Province · National Youth Talent Support Progra

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