Carbon Nanotubes Activate Limulus Amebocyte Lysate Coagulation by Interface Adsorption
Source abstract
Abstract
Limulus amebocyte lysate (LAL) assay is worldwide requested in the assessment of endotoxin contamination for biomaterials. As carbon nanotubes are one major nanomaterial with multiple potentials in biomedical application, here we investigate whether oxidized multiwalled carbon nanotubes (O-MWCNT) interferes the assessment by LAL assays. We showed that the endotoxin free O-MWCNT dispersing in aqueous solutions could activate both the gel-clotting and the end-point chromogenic LAL assay by converting coagulogen into coagulin through interfacial interactions between O-MWCNT and enzymes in the assays. In conclusion, the O-MWCNT could induce false positive results by activating the enzyme cascade of LAL.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Graphene and Nanomaterials Applications · Nanoparticles: synthesis and applications · Microplastics and Plastic Pollution
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Public Health, Capital Medical University, Beijing 100069, P. R. China
Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College, Beijing 100005, P. R. China
Funding
Ministry of Education of the People's Republic of China · Natural Science Foundation of Beijing Municipality · Chinese Academy of Medical Sciences · Beijing Key Laboratory of Environmental Toxicology, Capital Medical University · Youth Fund, Peking Union Medical College
References (25)
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