Rapid Evaluation of the Antibacterial Activity of Arylene–Ethynylene Compounds

A series of oligo(arylene-ethynylene) (1-3 repeat units) compounds functionalized with quaternary ammonium groups was screened for their antibacterial activity in the dark and with activation by long-wavelength (365 nm) UV irradiation. Several of these compounds have effective bactericidal activity (>99.9% killing) at concentrations between 0.01 and 10 μg/mL. Our approach uses flow cytometry to rapidly screen and evaluate the susceptibility of bacterial populations. The rapidity, high information content, and accuracy of this approach make it an extremely valuable method for the study of antibacterial compounds.