DataVXACS Applied Materials & InterfacesPaper
journal article·2025-12-10Open access

Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights

Omur Besbinar, Recep Uyar, Emel Kirbas Cilingir, Ana Martín-Pardillos, Jose L. Hueso, Ahmet Ceylan, Ozge Ozgenc, Okan Ekim, Mehmet Altay Unal, Fikret Ari, Roger M. Leblanc, Jesus Santamaria, Acelya Yilmazer

ACS Applied Materials & Interfaces· Volume 17· Issue 49
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Source abstract

Abstract

Exosomes, nanoscale extracellular vesicles, have emerged as promising carriers in drug delivery due to their ability to bypass biological barriers, low toxicity, high stability, and intrinsic targeting capabilities. Mesenchymal stem-cell-derived exosomes (EXO MSC ), with their natural tropism toward the tumor microenvironment, offer an ideal platform for enhancing therapeutic cargo delivery. In this study, we demonstrate an approach where red-emission chlorophyll-based carbon dots (Chl-CDs) were encapsulated within EXO MSC through a cell-driven uptake mechanism, creating CD@EXO MSC . These exosomes achieved superior photodynamic therapy (PDT) efficacy, requiring 40 times less nanomaterial compared to freestanding Chl-CDs. Mechanistic insights from glioblastoma cell miRNA profiling revealed that the enhanced efficacy was mediated by the regulation of efflux transporter genes, oxidative stress responses, and endocytosis pathways. This work highlights the synergistic potential of combining photosensitizers and miRNA-rich exosomes to achieve targeted and sustained therapeutic delivery, paving the way for a multifaceted approach in cancer therapy.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Extracellular vesicles in disease · Nanoplatforms for cancer theranostics · Carbon and Quantum Dots Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Stem Cell Institute

Ankara University

The Graduate School of Health Sciences of Ankara University

Department of Anatomy, Faculty of Veterinary Medicine

Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, Florida 33146, United States

Instituto de Nanociencia y Materiales de Aragon (INMA), CSIC-Universidad de Zaragoza, Campus Rio Ebro, Edificio I+D, C/Poeta Mariano Esquillor, s/n, 50018 Zaragoza, Spain

Department of Chemical Engineering and Environmental Technology (IQTMA)

University of Zaragoza

Networking Research Center in Biomaterials

Bioengineering and Nanomedicine (CIBER-BBN), Instituto de Salud Carlos III

Instituto de Investigación Sanitaria (IIS) de Aragón, Avenida San Juan Bosco, 13, 50009 Zaragoza, Spain

Escuela Politécnica Superior, Universidad de Zaragoza, Crta. de Cuarte s/n, 22071 Huesca, Spain

Department of Histology Embryology, Faculty of Veterinary Medicine

Department of Electrical Electronic Engineering

Faculty of Engineering

Department of Biomedical Engineering, Faculty of Engineering

Funding

Gobierno de Aragón · Ministerio de Ciencia, Innovación y Universidades · Ministerio de Economía y Competitividad · Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina · European Commission · Yüksekögretim Kurulu · Türkiye Bilimsel ve Teknolojik Arastirma Kurumu

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