journal article·2025-11-19

In Vivo SELEX: A Strategy for Identifying Ovarian Cancer-Specific Aptamers

Chanyuan Li, Weichang Chen, Zheng Zhang, Fensheng Qiu, Ruibin Jiang, Xiaona Fang, Siru Zhang, Longjie Li, Guomiao Liao, Yuanming Shen, Huarong Tang, Hui Meng, Tao Zhu, Zhaofeng Luo, Weihong Tan

ACS Applied Materials & Interfaces· Volume 17· Issue 46
Publisher page

Abstract

Ovarian cancer is a significant and life-threatening malignancy among women worldwide. Selective tumor targeting by molecular agents is essential for the development of effective therapeutics and diagnostics. Recognizing the disparities between in vivo and in vitro environments, we have implemented an in vivo SELEX strategy to identify DNA aptamers capable of specifically localizing to ovarian tumors in situ. Aptamer L1 has emerged as a promising candidate with a high binding affinity to Insulin-like Growth Factor Binding Protein 3 (IGFBP3), a tumor-promoting protein overexpressed in ovarian cancer. Notably, aptamer L1 effectively downregulates IGFBP3 expression and suppresses ovarian cancer cell proliferation. The unique molecular characteristics of aptamer L1 underscore its potential as a theranostic agent for ovarian cancer.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Nuclear Structure and Function · DNA and Nucleic Acid Chemistry

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Gynecologic Oncology, Women’s Hospital

Zhejiang University School of Medicine

The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM)

Chinese Academy of Sciences

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province

Hunan University

Wenzhou Medical University

Department of Basic Medicine

Anhui Medical College

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine

Sun Yat-Sen University Cancer Center

School of Science

Harbin Institute of Technology

Chongqing Taiji Industry (Group) Co. Ltd

Funding

Key Research and Development Program of Zhejiang Province · Social Development Project of Public Welfare Technology Research in Zhejiang Province

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