DataVXACS Applied Materials & InterfacesPaper
journal article·2023-06-07

Scalable and Uniform Fabrication of Dexamethasone-Eluting Depot-Engineered Stem Cell Spheroids as a Microtissue Construct to Target Bone Regeneration

Tiep Tien Nguyen, Mai Thi Phuong, Manju Shrestha, Junhyeung Park, Pham Le Minh, Jong Oh Kim, Hyukjae Choi, Joo-Won Nam, Hu-Lin Jiang, Hyung-Sik Kim, Jee-Heon Jeong, Jun-Beom Park, Simmyung Yook

ACS Applied Materials & Interfaces· Volume 15· Issue 22
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Source abstract

Abstract

Potentiation of stem cell potency is critical for successful tissue engineering, especially for bone regeneration. Three-dimensional cell culture and bioactive molecule co-delivery with cells have been proposed to achieve this effect. Here, we provide a uniform and scalable fabrication of osteogenic microtissue constructs of mesenchymal stem cell (MSC) spheroids surface-engineered with dexamethasone-releasing polydopamine-coated microparticles (PD-DEXA/MPs) to target bone regeneration. The microparticle conjugation process was rapid and cell-friendly and did not affect the cell viability or key functionalities. The incorporation of DEXA in the conjugated system significantly enhanced the osteogenic differentiation of MSC spheroids, as evidenced by upregulating osteogenic gene expression and intense alkaline phosphatase and alizarin red S staining. In addition, the migration of MSCs from spheroids was tested on a biocompatible macroporous fibrin scaffold (MFS). The result showed that PD-DEXA/MPs were stably anchored on MSCs during cell migration over time. Finally, the implantation of PD-DEXA/MP-conjugated spheroid-loaded MFS into a calvarial defect in a mouse model showed substantial bone regeneration. In conclusion, the uniform fabrication of microtissue constructs containing MSC spheroids with drug depots shows a potential to improve the performance of MSCs in tissue engineering.
Source: openalex_inverted_index · Quality: baseline

Research context

Biomaterials

OpenAlex topics: 3D Printing in Biomedical Research · Advanced Drug Delivery Systems · Graphene and Nanomaterials Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

College of Pharmacy, Keimyung University, Daegu 42601, Republic of Korea

College of Pharmacy, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of Korea

Department of Precision Medicine, School of Medicine, Sungkyunkwan University, Suwon 16419, Republic of Korea

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China

Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, China Pharmaceutical University, Nanjing 210009, China

Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, China Pharmaceutical University, Nanjing 210009, China

NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients, China Pharmaceutical University, Nanjing 210009, China

Department of Life Science in Dentistry, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea

Dental and Life Science Institute, Pusan National University, Yangsan 50612, Republic of Korea

Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea

Funding

Ministry of Health and Welfare · Ministry of Science and ICT, South Korea · National Research Foundation of Korea

References (55)

Show source-provided references
1. 10.1126/scitranslmed.aba456410.1126/scitranslmed.aba4564 ↗
2. 10.1016/j.ejcb.2019.04.00210.1016/j.ejcb.2019.04.002 ↗
3. 10.3727/096368915X68962210.3727/096368915X689622 ↗
4. 10.1038/s41536-019-0083-610.1038/s41536-019-0083-6 ↗
5. 10.1002/sctm.19-020210.1002/sctm.19-0202 ↗
6. 10.1155/2019/962853610.1155/2019/9628536 ↗
7. 10.1126/sciadv.abn861410.1126/sciadv.abn8614 ↗
8. 10.1002/btm2.1015810.1002/btm2.10158 ↗
9. 10.3390/molecules2403060310.3390/molecules24030603 ↗
10. 10.1007/s40005-022-00568-w10.1007/s40005-022-00568-w ↗
11. 10.3389/fbioe.2020.0037310.3389/fbioe.2020.00373 ↗
12. 10.1007/s13206-018-3101-710.1007/s13206-018-3101-7 ↗
13. 10.1016/j.bioactmat.2016.05.00110.1016/j.bioactmat.2016.05.001 ↗
14. 10.1089/ten.tea.2018.023710.1089/ten.tea.2018.0237 ↗
15. 10.1007/10_2011_11510.1007/10_2011_115 ↗
16. 10.1016/j.msec.2020.11110210.1016/j.msec.2020.111102 ↗
17. 10.1007/s40005-019-00439-x10.1007/s40005-019-00439-x ↗
18. 10.3389/fcell.2019.0019310.3389/fcell.2019.00193 ↗
19. 10.1016/j.actbio.2017.08.00310.1016/j.actbio.2017.08.003 ↗
20. 10.1016/j.ijpharm.2017.02.01410.1016/j.ijpharm.2017.02.014 ↗
21. 10.1080/15548627.2020.185060810.1080/15548627.2020.1850608 ↗
22. 10.1016/j.biomaterials.2010.12.03610.1016/j.biomaterials.2010.12.036 ↗
23. 10.1021/acs.bioconjchem.6b0054410.1021/acs.bioconjchem.6b00544 ↗
24. 10.1016/j.biomaterials.2019.11941510.1016/j.biomaterials.2019.119415 ↗
25. 10.1186/scrt32810.1186/scrt328 ↗
26. 10.1016/j.biomaterials.2007.08.02010.1016/j.biomaterials.2007.08.020 ↗
27. 10.1016/j.biomaterials.2008.06.03010.1016/j.biomaterials.2008.06.030 ↗
28. 10.1016/j.yexcr.2013.12.01710.1016/j.yexcr.2013.12.017 ↗
29. 10.2147/IJN.S12467110.2147/IJN.S124671 ↗
30. 10.1007/s40005-021-00551-x10.1007/s40005-021-00551-x ↗
31. 10.1007/s40005-021-00554-810.1007/s40005-021-00554-8 ↗
32. Ciuffreda M. C. · 2016 · 1416 · 14910.1007/978-1-4939-3584-0_8 ↗
33. 10.1016/j.jconrel.2021.06.02010.1016/j.jconrel.2021.06.020 ↗
34. 10.1021/acsami.7b1986510.1021/acsami.7b19865 ↗
35. 10.1007/s00441-014-1830-z10.1007/s00441-014-1830-z ↗
36. 10.1186/s13287-017-0558-610.1186/s13287-017-0558-6 ↗
37. 10.1016/j.biomaterials.2017.11.02310.1016/j.biomaterials.2017.11.023 ↗
38. 10.5966/sctm.2015-041210.5966/sctm.2015-0412 ↗
39. 10.1016/j.biomaterials.2019.11965210.1016/j.biomaterials.2019.119652 ↗
40. 10.1016/j.bbrc.2019.12.06610.1016/j.bbrc.2019.12.066 ↗
41. 10.1088/1758-5090/abd56c10.1088/1758-5090/abd56c ↗
42. 10.1016/j.biomaterials.2019.04.00110.1016/j.biomaterials.2019.04.001 ↗
43. 10.1007/s40005-017-0382-010.1007/s40005-017-0382-0 ↗
44. 10.1089/ten.teb.2015.035710.1089/ten.teb.2015.0357 ↗
45. 10.1021/acsabm.9b0098910.1021/acsabm.9b00989 ↗
46. 10.1016/j.bsbt.2016.11.00110.1016/j.bsbt.2016.11.001 ↗
47. 10.2217/nnm.11.7610.2217/nnm.11.76 ↗
48. 10.1038/srep3737110.1038/srep37371 ↗
49. 10.1016/j.jconrel.2020.02.02910.1016/j.jconrel.2020.02.029 ↗
50. 10.1007/s40005-014-0156-x10.1007/s40005-014-0156-x ↗
51. 10.3727/096368910X56407610.3727/096368910X564076 ↗
52. 10.1038/s41419-021-03839-w10.1038/s41419-021-03839-w ↗
53. 10.1038/srep1668110.1038/srep16681 ↗
54. 10.1691/ph.2016.600910.1691/ph.2016.6009 ↗
55. 10.3390/polym303137710.3390/polym3031377 ↗