DataVXACS Applied Materials & InterfacesPaper
journal article·2020-12-30

Developing Protein–Antitumoral Drug Nanoconjugates as Bifunctional Antimicrobial Agents

Naroa Serna, Jose Vicente Carratalá, Eloi Parladé, Alejandro Sánchez-Chardi, Anna Aviñó, Ugutz Unzueta, Ramón Mangues, Ramón Eritja, Neus Ferrer-Miralles, Esther Vazquez, Antonio Villaverde

ACS Applied Materials & Interfaces· Volume 12· Issue 52
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Source abstract

Abstract

A novel concept about bifunctional antimicrobial drugs, based on self-assembling protein nanoparticles, has been evaluated here over two biofilm-forming pathogens, namely Pseudomonas aeruginosa and Staphylococcus aureus. Two structurally different antimicrobial peptides (GWH1 and PaDBS1R1) were engineered to form regular nanoparticles of around 35 nm, to which the small molecular weight drug Floxuridine was covalently conjugated. Both the assembled peptides and the chemical, a conventional cytotoxic drug used in oncotherapy, showed potent antimicrobial activities that were enhanced by the combination of both molecules in single pharmacological entities. Therefore, the resulting prototypes show promises as innovative nanomedicines, being potential alternatives to conventional antibiotics. The biological performance and easy fabrication of these materials fully support the design of protein-based hybrid constructs for combined molecular therapies, expected to have broad applicability beyond antimicrobial medicines. In addition, the approach taken here validates the functional exploration and repurposing of antitumoral drugs, which at low concentrations perform well as unexpected biofilm-inhibiting agents.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Antimicrobial Peptides and Activities · Biochemical and Structural Characterization · Bacteriophages and microbial interactions

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain

Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain

CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3-5, 28029 Madrid, Spain

Servei de Microscòpia, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain

Departament de Biologia Evolutiva, Ecologia i Ciències Ambientals, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain

Institute for Advanced Chemistry of Catalonia (IQAC), CSIC, 08034 Barcelona, Spain

Biomedical Research Institute Sant Pau (IIB Sant Pau), Sant Antoni Maria Claret 167, 08025 Barcelona, Spain

Funding

Ministerio de Ciencia e Innovaci?n · Centro de Investigaci?n Biom?dica en Red en Bioingenier?a, Biomateriales y Nanomedicina · European Regional Development Fund · Instituto de Salud Carlos III · European Social Fund · Ag?ncia de Gesti? d'Ajuts Universitaris i de Recerca · Agencia estatal de investigaci?n

References (67)

Show source-provided references
1. 10.1186/s12916-019-1314-910.1186/s12916-019-1314-9 ↗
2. 10.3390/molecules17101227610.3390/molecules171012276 ↗
3. 10.3389/fcimb.2016.0019410.3389/fcimb.2016.00194 ↗
4. 10.2174/138920102066619101112172210.2174/1389201020666191011121722 ↗
5. 10.1002/med.2154210.1002/med.21542 ↗
6. 10.3389/fchem.2019.0082410.3389/fchem.2019.00824 ↗
7. 10.1016/j.biotechadv.2020.10760310.1016/j.biotechadv.2020.107603 ↗
8. Lei J. · Am. J. Translational Res. · 2019 · 11 · 3919
9. 10.1038/s41587-019-0222-z10.1038/s41587-019-0222-z ↗
10. 10.3390/antibiotics901002410.3390/antibiotics9010024 ↗
11. 10.3390/nano1003056010.3390/nano10030560 ↗
12. 10.3389/fmicb.2019.0274010.3389/fmicb.2019.02740 ↗
13. 10.2174/156802662066620042709091210.2174/1568026620666200427090912 ↗
14. 10.3389/fmicb.2019.0116010.3389/fmicb.2019.01160 ↗
15. 10.1016/j.bbamem.2015.10.01310.1016/j.bbamem.2015.10.013 ↗
16. 10.1016/j.bbrc.2018.05.01110.1016/j.bbrc.2018.05.011 ↗
17. 10.1099/mic.0.025643-010.1099/mic.0.025643-0 ↗
18. 10.1080/20002297.2018.144208910.1080/20002297.2018.1442089 ↗
19. 10.3390/microorganisms802028010.3390/microorganisms8020280 ↗
20. 10.1007/s00280-016-3054-210.1007/s00280-016-3054-2 ↗
21. 10.1097/CCM.0b013e3181f265ba10.1097/CCM.0b013e3181f265ba ↗
22. 10.1016/j.bmcl.2016.04.03610.1016/j.bmcl.2016.04.036 ↗
23. 10.1111/lam.1321310.1111/lam.13213 ↗
24. 10.1016/j.tim.2017.10.00910.1016/j.tim.2017.10.009 ↗
25. 10.1186/s13045-020-00863-910.1186/s13045-020-00863-9 ↗
26. 10.1002/open.20190003810.1002/open.201900038 ↗
27. 10.15252/emmm.20170877210.15252/emmm.201708772 ↗
28. 10.1080/1525777950801466710.1080/15257779508014667 ↗
29. 10.1002/adfm.20170091910.1002/adfm.201700919 ↗
30. 10.1002/advs.20190242010.1002/advs.201902420 ↗
31. 10.1016/j.jconrel.2020.01.01910.1016/j.jconrel.2020.01.019 ↗
32. 10.7150/thno.4323110.7150/thno.43231 ↗
33. 10.1016/j.actbio.2020.06.03310.1016/j.actbio.2020.06.033 ↗
34. 10.1002/ppsc.20200004010.1002/ppsc.202000040 ↗
35. 10.1016/j.actbio.2017.07.02710.1016/j.actbio.2017.07.027 ↗
36. 10.1088/1361-6528/aaf95910.1088/1361-6528/aaf959 ↗
37. 10.1021/mp200394t10.1021/mp200394t ↗
38. 10.1021/nn405573210.1021/nn4055732 ↗
39. 10.1002/ppsc.20190030410.1002/ppsc.201900304 ↗
40. 10.2217/nnm.09.9810.2217/nnm.09.98 ↗
41. 10.1016/j.str.2013.08.00510.1016/j.str.2013.08.005 ↗
42. 10.1093/nar/gkh46810.1093/nar/gkh468 ↗
43. 10.1002/jcc.2008410.1002/jcc.20084 ↗
44. 10.1016/j.ijantimicag.2008.04.00310.1016/j.ijantimicag.2008.04.003 ↗
45. 10.1016/j.bbamem.2018.08.00110.1016/j.bbamem.2018.08.001 ↗
46. 10.1016/j.biomaterials.2012.08.03310.1016/j.biomaterials.2012.08.033 ↗
47. 10.1016/j.actbio.2018.10.03010.1016/j.actbio.2018.10.030 ↗
48. 10.1002/adma.20150367610.1002/adma.201503676 ↗
49. 10.1016/j.nano.2013.11.00610.1016/j.nano.2013.11.006 ↗
50. 10.1586/eri.13.1210.1586/eri.13.12 ↗
51. 10.3389/fmicb.2019.0053910.3389/fmicb.2019.00539 ↗
52. 10.1111/j.1751-7915.2008.00060.x10.1111/j.1751-7915.2008.00060.x ↗
53. 10.3390/antibiotics404042410.3390/antibiotics4040424 ↗
54. 10.1073/pnas.122270611010.1073/pnas.1222706110 ↗
55. 10.1038/s41564-019-0357-110.1038/s41564-019-0357-1 ↗
56. 10.3390/antibiotics804027010.3390/antibiotics8040270 ↗
57. 10.2174/138161282166615050615443410.2174/1381612821666150506154434 ↗
58. 10.1021/acs.biomac.9b0022810.1021/acs.biomac.9b00228 ↗
59. 10.1021/acs.biochem.9b0005510.1021/acs.biochem.9b00055 ↗
60. 10.1038/s41467-019-13491-w10.1038/s41467-019-13491-w ↗
61. 10.1002/wnan.157810.1002/wnan.1578 ↗
62. 10.1002/ijch.20190005110.1002/ijch.201900051 ↗
63. 10.1016/j.sbi.2020.05.00110.1016/j.sbi.2020.05.001 ↗
64. 10.1002/advs.20190242010.1002/advs.201902420 ↗
65. 10.1007/s40843-019-1231-y10.1007/s40843-019-1231-y ↗
66. 10.1002/smll.20200188510.1002/smll.202001885 ↗
67. 10.1016/j.tibs.2020.08.00310.1016/j.tibs.2020.08.003 ↗