DataVXACS Applied Materials & InterfacesPaper
journal article·2021-11-24

Fully Integrated Self-Powered Electrical Stimulation Cell Culture Dish for Noncontact High-Efficiency Plasmid Transfection

Miaomiao Yuan, Xiaoying Li, Jingfeng Liu, Youbin Zheng, Li Cheng, Ning Tang, Rongjun Zhang, Shuxiang Xu, Xuemei Fu, Hossam Haick, Yang Xu

ACS Applied Materials & Interfaces· Volume 13· Issue 46
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Source abstract

Abstract

Plasmid DNA transfection of mammalian cells is widely used in biomedical research and genetic drug delivery, but low transfection efficiency, especially in the context of the primary cells, limits its application. To improve the efficiency of plasmid transfection, a fully integrated self-powered electrical stimulation cell culture dish (SESD) has been developed to provide self-powered electrical stimulation (ES) of adherent cells, significantly improving the efficiency of plasmid transfection into mammalian cells and cell survival by the standard lipofectamine transfection method. Mechanistically, ES can safely increase the intracellular calcium concentration by opening calcium-ion channels, leading to a higher efficiency of plasmid transfection. Therefore, SESD has the potential to become an effective platform for high-efficiency plasmid DNA transfection in biomedical research and drug delivery.
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Research context

OpenAlex topics: Neuroscience and Neural Engineering · Microfluidic and Bio-sensing Technologies · 3D Printing in Biomedical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen 518033, China

School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Guangdong, China

Department of Rheumatism and Immunology, Peking University Shenzhen Hospital, Shenzhen 518036, China

Department of Chemical Engineering and Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa 3200003, Israel

School of Materials and Energy, Lanzhou University, Lanzhou 730000, China

The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China

Funding

Sun Yat-sen University · National Natural Science Foundation of China · Shenzhen ???Sanming??? Project of Medicine

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