DataVXACS Applied Materials & InterfacesPaper
journal article·2019-05-22

Preparation and Properties of a Low Fouling Magnetic Nanoparticle and Its Application to the HPV Genotypes Assay in Whole Serum

Lihua Chen, Mingchao Liu, Yan Tang, Chuangfu Chen, Xingxing Wang, Zhiqiang Hu

ACS Applied Materials & Interfaces· Volume 11· Issue 20
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Source abstract

Abstract

Gold magnetic particles as a new carrier of disease diagnosis probes have attracted wide attention but encountered a bottleneck. That is, the interfacial properties of gold magnetic particles are susceptible to the influence of nonspecific biological molecules in actual diagnostic samples. Here, a novel nanoparticle made by the covalent attachment of polyethyleneimine and hyperbranched polyether polyol onto the gold shell surface of a magnetic bead demonstrated not only low fouling properties but also excellent stability in a variety of external environments, especially in complex biological systems. Most importantly, in its application as the probe for sensitive and selective fluorescence detection of high-risk human papillomavirus (HPV) genotypes 18, 16 in buffer, even in 100% serum, a good linear correlation with the concentration of HPV18/16 target DNA ranging from 5 nM to 1 μM was shown with the low detection limits. To our knowledge, this is one of the few successful examples of direct application of magnetic beads to the detection of disease markers in whole serum, suggesting that this material has good commercial potential and value.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Biosensors and Analytical Detection · Advanced biosensing and bioanalysis techniques · Advanced Biosensing Techniques and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, Ministry of Education; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, Shandong Province; Key Laboratory of Eco-Chemical Engineering; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China

Key Laboratory of Prevention and Control of Animal Disease of Xinjiang Corps. College of Animal Science and Technology, Shihezi University, 832000 Shihezi, Xinjiang, P. R. China

Funding

Ministry of Science and Technology of the People's Republic of China · Xinjiang autonomous region · Science and technology branch project of Xinjiang autonomous region · The Applied Basic Research Program of Qingdao · Science and technology project and achievement transformation plan of modern agricultural of Xinjiang Corps

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