DataVXACS Applied Materials & InterfacesPaper
journal article·2021-04-21

Electrochemical Trans-Channel Assay for Efficient Evaluation of Tumor Cell Invasiveness

Liu Shi, Lin Wang, Xuemei Ma, Mohammed Jalalah, Saeed A. Alsareii, Tao Gao, Farid A. Harraz, Genxi Li

ACS Applied Materials & Interfaces· Volume 13· Issue 15
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Abstract

Efficiently assessing the invasive capability of tumor cells is critical both for the research and treatment of cancer. Here, we report a novel method called the electrochemical trans-channel assay for efficient evaluation of tumor cell invasiveness. A bioinspired extracellular matrix degradation model (EDM) has been first fabricated on a porous anodic alumina (PAA) membrane to construct the electrochemical apparatus. Upon contacting the invasive tumor cells, invasive capability can be sensitively evaluated by the degree of EDM impairment, which is recorded by the electrochemical trans-channel ionic currents in a label-free manner. Compared to the most commonly used trans-well migration method, this assay can be accomplished in an efficient way that is significantly faster (20 min) and more convenient. Besides, quantitation can also be realized for monitoring the invasion process, which cannot be achieved by other currently used methods. Our proposed electrochemical trans-channel assay method has shown a synergistic effect for the evaluation of tumor cell invasiveness, providing a promising method for clinical assessment or prognostic applications of tumor metastasis.
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Research context

OpenAlex topics: Anodic Oxide Films and Nanostructures · Advanced biosensing and bioanalysis techniques · Semiconductor materials and devices

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P. R. China

Promising Centre for Sensors and Electronic Devices (PCSED), Advanced Materials and Nano-Research Centre, Najran University, P.O. Box: 1988, Najran 11001, Saudi Arabia

Department of Electrical Engineering, Faculty of Engineering, Najran University, Najran, Saudi Arabia

Department of Surgery, College of Medicine, Najran University, Najran, Saudi Arabia

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, P. R. China

Nanomaterials and Nanotechnology Department, Central Metallurgical Research and Development Institute (CMRDI), P.O. 87 Helwan, Cairo 11421, Egypt

Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, P. R. China

Funding

Natural Science Foundation of Jiangsu Province · Government of Jiangsu Province · National Natural Science Foundation of China · Ministry of Education in Saudi Arabia

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