DataVXACS Applied Materials & InterfacesPaper
journal article·2017-05-03

GPCR Activation and Endocytosis Induced by a 2D Material Agonist

Wei-Tao Dou, Ya Kong, Xiao-Peng He, Guo-Rong Chen, Yi Zang, Jia Li, He Tian

ACS Applied Materials & Interfaces· Volume 9· Issue 17
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Source abstract

Abstract

Agonist-induced activation and endocytosis of G protein-coupled receptors (GPCRs) are crucial for a number of physiological and pathological processes. However, tools that are available for probing GPCR endocytosis have been insufficient. Here, we developed a two-dimensional (2D) material agonist by supramolecular self-assembly between an endogenous agonist of κ-opioid receptor (KOR) and 2D molybdenum disulfide. The 2D material agonist has proven to be amenable for eliciting GPCR activation and endocytosis in cells stably expressing KOR rather than in those without KOR expression. Using super-resolution microscopy, we also show that the 2D material agonist colocalizes well with GFP-fused KOR intracellularly. Further, the endocytosed 2D material agonist can selectively produce reactive oxygen species in cells that overly express KOR, as controlled by light irradiation.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Neuropeptides and Animal Physiology · 2D Materials and Applications · Luminescence and Fluorescent Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Key Laboratory for Advanced Materials & Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China

National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 189 Guo Shoujing Road, Shanghai 201203, P. R. China

University of Chinese Academy of Sciences, UCAS, No. 19A, Yuquan Road, Beijing 100049, P. R. China

Funding

Ministry of Education of the People's Republic of China · Ministry of Science and Technology of the People's Republic of China · Science and Technology Commission of Shanghai Municipality · National Natural Science Foundation of China · Shanghai Science and Technology Development Funds

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