journal article·2021-05-05

Immunostimulatory AIE Dots for Live-Cell Imaging and Drug Delivery

Peilin Gu, Bin Chen, Tingting Zhai, Qian Li, Xiaolei Zuo, Lihua Wang, Anjun Qin, Yi Zhou, Jianlei Shen

ACS Applied Materials & Interfaces· Volume 13· Issue 17
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Abstract

The mechanical properties of nanoscale drug carriers play critical roles in regulating nano-bio interactions. For example, the superior deformability of the softer nanoparticles enables them to pass through the biofilters efficiently, facilitating their long blood circulation and better tumor penetration. However, as a novel nanocarrier system, the elimination efficiency of soft nanoparticles from cells is poorly investigated. Here, we report a facile strategy to prepare soft luminescent nanoparticles through self-assembly of amphiphilic aggregation-induced emission (AIE) fluorophores. The prepared soft AIE dots exhibit strong light emission (quantum yield, ∼27.1%) and can reveal the encapsulation and excretion process of NPs in real time. The cell results showed that soft NPs can greatly increase the transfer speed of nanomaterials into cells and accelerate their elimination from cells through the sacrifice of soft AIE dots. We also show that soft AIE dots loaded with cytosine-phosphate-guanine (CpG) oligodeoxynucleotides can induce strong immunostimulatory effects, producing a high level of various proinflammatory cytokines including tumor necrosis factor (TNF)-R, interleukin (IL)-6, and IL-12. This work demonstrates a new design strategy for synthesizing a soft nanocarrier system that can deliver drugs into cells efficiently and then be eliminated from cells quickly.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Luminescence and Fluorescent Materials · Nanoplatforms for cancer theranostics · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

University of Chinese Academy of Sciences, Beijing 100049, China

Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acids Chemistry and Nanomedicine State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China

Bioimaging Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China

State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou 510640, China

College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China

Funding

Science and Technology Commission of Shanghai Municipality · National Natural Science Foundation of China

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