journal article·2020-06-17Open access

Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography

T. Sansen, D. Sanchez-Fuentes, R. Rathar, A. Colom-Diego, F. El Alaoui, J. Viaud, M. Macchione, S. de Rossi, S. Matile, R. Gaudin, V. Bäcker, A. Carretero-Genevrier, L. Picas

Abstract

Membrane shape is a key feature of many cellular processes, including cell differentiation, division, migration, and trafficking. The development of nanostructured surfaces allowing for the in situ manipulation of membranes in living cells is crucial to understand these processes, but this requires complicated and limited-access technologies. Here, we investigate the self-organization of cellular membranes by using a customizable and benchtop method allowing one to engineer 1D SiO2 nanopillar arrays of defined sizes and shapes on high-performance glass compatible with advanced microscopies. As a result of this original combination, we provide a mapping of the morphology-induced modulation of the cell membrane mechanics, dynamics and steady-state organization of key protein complexes implicated in cellular trafficking and signal transduction.
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Research context

OpenAlex topics: Nanofabrication and Lithography Techniques · Microfluidic and Bio-sensing Technologies · Force Microscopy Techniques and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS UMR 9004−Université de Montpellier, 34293 Montpellier, France

Institut d’Électronique et des Systèmes (IES), CNRS UMR 5214−Université de Montpellier, 34097 Montpellier, France

Biochemistry Department and School of Chemistry and Biochemistry and Swiss National Centre for Competence in Research in Chemical Biology, University of Geneva, CH-1211 Geneva, Switzerland

Institute of Cardiovascular and Metabolic Diseases (I2MC-UMR1048), Inserm and Université Toulouse 3, Avenue Jean Poulhès BP84225, 31432 Cedex 04 Toulouse, France

School of Chemistry and Biochemistry and Swiss National Centre for Competence in Research in Chemical Biology, University of Geneva, CH-1211 Geneva, Switzerland

MRI Imaging Facility, UMS BioCampus Montpellier, 34000 Montpellier, France

Funding

Agence Nationale de la Recherche · Institut National de la Sant? et de la Recherche M?dicale · Centre National de la Recherche Scientifique · H2020 European Research Council

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