journal article·2017-09-27

Clickable Multifunctional Dumbbell Particles for in Situ Multiplex Single-Cell Cytokine Detection

Peng Zhao, Justin George, Bin Li, Nooshin Amini, Janet Paluh, Jun Wang

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Abstract

We report a novel strategy for fabrication of multifunctional dumbbell particles (DPs) through click chemistry for monitoring single-cell cytokine releasing. Two different types of DPs were prepared on a large scale through covalent bioorthogonal reaction between methyltetrazine and trans-cyclooctene on a microchip under a magnetic field. After collection of the DPs, the two sides of each particle were further functionalized with different antibodies for cell capturing and cytokine detection, respectively. These DPs labeled with different fluorescent dyes have been used for multiplex detection and analysis of cytokines secreted by single live cells. Our results show that this new type of DPs are promising for applications in cell sorting, bioimaging, single-cell analysis, and biomedical diagnostics.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Biosensors and Analytical Detection · Advanced biosensing and bioanalysis techniques · Advanced Biosensing Techniques and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Multiplex Biotechnology Laboratory, Department of Chemistry, University at Albany, State University of New York, Albany, New York 12222, United States

College of Nanoscale Science and Engineering, State University of New York Polytechnic Institute, Albany, New York 12203, United States

Cancer Research Center, University at Albany, State University of New York, Rensselaer, New York 12144, United States

Funding

University at Albany · Presidential Initiatives Fund for Research and Scholarship, University at Albany

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