journal article·2019-02-20

Gold Nanoparticle Probe-Assisted Antigen-Counting Chip Using SEM

Xin Zhou, Chih-Tsung Yang, Qiaoshu Xu, Zhichao Lou, Zhengfeng Xu, Benjamin Thierry, Ning Gu

Publisher page

Abstract

Currently, it remains challenging to count protein-biomarker molecules present in a small droplet of biological samples. Herein, we propose a gold nanoparticle (GNP) probe-assisted sandwich-counting strategy that relies on a GNP probe, an antibody-functionalized chip to "count" antigen molecules using a scanning electron microscope. Both standard carcinoembryonic antigen (CEA) and two real CEA-related tumor samples (tumor tissues and serum) were assayed to demonstrate the proof-of-concept of the counting strategy. Results show that our method is excellently correlative with enzyme-linked immuno-sorbent assay (ELISA) that is widely used in clinics for antigen or antibody detection and the limit of detection of our enumeration strategy reaches down to 0.045 ng/mL, which is ∼40 times more sensitive than the conventional ELISA. Therefore, our GNP probe-assisted sandwich-counting strategy has the potential to be used for quantification of protein biomarkers at ultralow concentrations in early tumor specimens and detection of target proteins in much diluted concentrations.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Advanced Biosensing Techniques and Applications · Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Comparative Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China

Future Industries Institute and ARC Centre of Excellence in Convergent Bio and Nano Science and Technology, Mawson Lakes Campus, University of South Australia, South Australia 5095, Australia

State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210009, China

College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China

Center of Medical Genetics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China

Funding

Yangzhou University · National Natural Science Foundation of China · Priority Academic Program Development of Jiangsu Higher Education Institution

References (33)

Show source-provided references
1. 10.1016/s0731-7085(99)00244-710.1016/s0731-7085(99)00244-7 ↗
2. 10.1038/nature0818410.1038/nature08184 ↗
3. 10.1021/pr101070j10.1021/pr101070j ↗
4. 10.1021/ac400051410.1021/ac4000514 ↗
5. 10.1073/pnas.132399811110.1073/pnas.1323998111 ↗
6. 10.1021/am506344j10.1021/am506344j ↗
7. 10.1021/acsami.5b0544310.1021/acsami.5b05443 ↗
8. 10.1039/c5lc01558h10.1039/c5lc01558h ↗
9. 10.1021/ja102271r10.1021/ja102271r ↗
10. 10.1038/nmat437710.1038/nmat4377 ↗
11. Gaskin, J.; Abbott, T.; Medley, S.; Patty, K.; Gregory, D.; Thaisen, K.; Ramsey, B.; Jerman, G.; Sampson, A.; Harvey, R.; Taylor, L. Mimature Scanning Electron Microscope for in-Situ Planetary Studies. Earth and Space 2010: Engineering ,Sciecne, Construction, and Operation in Challenging Environments, 2010; p 1246.10.1061/41096(366)113 ↗
12. 10.1016/j.mee.2006.01.02310.1016/j.mee.2006.01.023 ↗
13. 10.1017/s143192761500615710.1017/s1431927615006157 ↗
14. 10.1093/nar/28.12.e6610.1093/nar/28.12.e66 ↗
15. 10.1016/s0167-5699(00)01683-210.1016/s0167-5699(00)01683-2 ↗
16. 10.1126/science.289.5485.176010.1126/science.289.5485.1760 ↗
17. 10.1016/s0022-1759(01)00325-810.1016/s0022-1759(01)00325-8 ↗
18. 10.1080/1052029000906843310.1080/10520290009068433 ↗
19. 10.1006/jsbi.1994.100410.1006/jsbi.1994.1004 ↗
20. 10.1021/ac011127p10.1021/ac011127p ↗
21. 10.1038/bjc.2012.60510.1038/bjc.2012.605 ↗
22. 10.1016/0378-4347(91)80252-810.1016/0378-4347(91)80252-8 ↗
23. 10.1016/s0956-5663(99)00043-310.1016/s0956-5663(99)00043-3 ↗
24. 10.1103/physrevlett.56.93010.1103/physrevlett.56.930 ↗
25. 10.1021/am402302r10.1021/am402302r ↗
26. 10.1021/acssensors.6b0077610.1021/acssensors.6b00776 ↗
27. 10.1039/c2an16257a10.1039/c2an16257a ↗
28. 10.1039/c6tc03981b10.1039/c6tc03981b ↗
29. Hsieh J.-F. · J. Food Drug Anal. · 2010 · 18 · 434
30. 10.1186/s40425-018-0394-y10.1186/s40425-018-0394-y ↗
31. 10.3748/wjg.v18.i17.212110.3748/wjg.v18.i17.2121 ↗
32. 10.1159/00044706210.1159/000447062 ↗
33. 10.1126/scitranslmed.aah558310.1126/scitranslmed.aah5583 ↗