DMSA-Functionalized Mesoporous Alumina with a High Capacity for Selective Isolation of Immunoglobulin G

A novel dimercaptosuccinic acid-functionalized mesoporous alumina (DMSA–MA) is synthesized by the dicarboxylic acid groups of dimercaptosuccinic acid molecules coordinating to the Al3+ ions located in the mesostructure. The as-prepared DMSA–MA composites possess a large surface area of 91.17 m2/g as well as a uniform pore size and a high pore volume of 17.22 nm and 0.23 cm3/g, respectively. DMSA coating of mesostructures significantly enhanced their selectivity for glycoprotein adsorption through a powerful hydrophilic binding force, and the maximum adsorption capacity of immunoglobulin G (IgG) can reach 2298.6 mg g–1. The captured IgG could be lightly stripped from the DMSA–MA composites with an elution rate of 98.3% by using 0.5 wt % CTAB solution as the elution reagent. DMSA–MA is further employed as a sorbent for the enrichment of IgG heavy chain and light chain from human serum sample. SDS–PAGE assay results showed the obtained IgG with high purity compared to that of the standard solution of IgG.