Synergistic Photothermal Ice Inhibition Strategy for Scalable Cryopreservation of Macroscopic Spheroids and Immune Cells in Advanced Immunotherapy
Source abstract
Abstract
Scalable and effective cryopreservation of human immune cells and biological samples is challenging for advanced cellular therapeutics. However, current cryoprotectants are limited due to trade-offs between robust ice inhibition and uniform thermal management to mitigate Ostwald ripening. Herein, we develop a nonpermeable nanocryoprotectant (PVA@PPy) by combining antifreezing poly(vinyl alcohol) (PVA) with photothermal polypyrrole (PPy). The strong hydration of pendant PVA chains limits ice growth, while the photothermal PPy enables bulky heating to prevent devitrification. A low concentration of 0.05% nanocryoprotectant enables the recovery of 0.5 mm neural spheroids, outperforming traditional cryopreservation methods employing 10% DMSO. We achieve >95% recovery rates across all tested human immune cell populations, including CD4+, CD8+, NK cells, and αβ T cells. This work underscores the unprecedented potential of PVA@PPy for the effective preservation of macroscopic biological samples and immune cells for advanced therapeutics.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Solar-Powered Water Purification Methods · Nanoplatforms for cancer theranostics · RNA Interference and Gene Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Biopharmaceutical and Health Engineering, Key Laboratory of Active Proteins and Peptides Green Biomanufacturing of Guangdong Higher Education Institutes, Shenzhen International Graduate School
Tsinghua University
Department of Hepatopancreatobiliary Surgery, Beijing Tsinghua Changgeng Hospital
School of Energy and Mechanical Engineering
Nanjing Normal University
Centre for Advanced Macromolecular Design and Australian Centre for Nanomedicine, School of Chemical Engineering
University of New South Wales
Funding
Tsinghua Shenzhen International Graduate School · Guangdong Provincial Pearl River Talents Program · Science, Technology and Innovation Commission of Shenzhen Municipality · National Natural Science Foundation of China · Scientific Research Foundation from Shenzhen Finance Bureau · General Program of Guangdong Province
References (59)
Show source-provided references
1. 10.1016/j.bioactmat.2024.09.00810.1016/j.bioactmat.2024.09.008 ↗
2. 10.1002/adfm.20230337310.1002/adfm.202303373 ↗
3. 10.1038/s41467-023-38824-810.1038/s41467-023-38824-8 ↗
4. 10.51335/organoid.2023.3.e1510.51335/organoid.2023.3.e15 ↗
5. 10.1002/biot.20230054310.1002/biot.202300543 ↗
6. 10.1016/j.molmed.2023.12.00410.1016/j.molmed.2023.12.004 ↗
7. 10.1021/acssuschemeng.2c0157810.1021/acssuschemeng.2c01578 ↗
8. 10.1186/s12951-025-03265-610.1186/s12951-025-03265-6 ↗
9. 10.1126/sciadv.abe300510.1126/sciadv.abe3005 ↗
10. 10.1002/advs.20200242510.1002/advs.202002425 ↗
11. 10.1016/j.actbio.2022.10.05610.1016/j.actbio.2022.10.056 ↗
12. 10.1016/j.actbio.2021.07.00110.1016/j.actbio.2021.07.001 ↗
13. 10.3390/bioengineering1005050810.3390/bioengineering10050508 ↗
14. 10.1002/adma.20200851710.1002/adma.202008517 ↗
15. 10.1038/s41570-022-00407-410.1038/s41570-022-00407-4 ↗
16. 10.3389/fmed.2020.59224210.3389/fmed.2020.592242 ↗
17. 10.1002/adbi.20220028510.1002/adbi.202200285 ↗
18. 10.1038/s41467-021-21717-z10.1038/s41467-021-21717-z ↗
19. 10.1021/jacs.9b1294310.1021/jacs.9b12943 ↗
20. 10.1016/j.cryobiol.2023.10478610.1016/j.cryobiol.2023.104786 ↗
21. 10.7150/thno.6753010.7150/thno.67530 ↗
22. 10.1021/acs.biomac.2c0112010.1021/acs.biomac.2c01120 ↗
23. 10.1038/s41467-017-01421-710.1038/s41467-017-01421-7 ↗
24. 10.3390/cells1112185610.3390/cells11121856 ↗
25. 10.1007/s10439-023-03255-510.1007/s10439-023-03255-5 ↗
26. 10.1080/02656736.2022.202890810.1080/02656736.2022.2028908 ↗
27. 10.1021/nn201560b10.1021/nn201560b ↗
28. 10.1021/acs.chemrev.3c0015910.1021/acs.chemrev.3c00159 ↗
29. 10.1002/adma.20120221110.1002/adma.201202211 ↗
30. 10.1016/j.cej.2018.11.08810.1016/j.cej.2018.11.088 ↗
31. 10.1002/pola.2632810.1002/pola.26328 ↗
32. 10.1038/s41598-024-61587-110.1038/s41598-024-61587-1 ↗
33. 10.1021/jp107435b10.1021/jp107435b ↗
34. 10.1007/s10854-019-01109-210.1007/s10854-019-01109-2 ↗
35. 10.1038/s41598-019-44900-110.1038/s41598-019-44900-1 ↗
36. 10.1089/bio.2023.001510.1089/bio.2023.0015 ↗
37. 10.1016/j.matpr.2019.06.56610.1016/j.matpr.2019.06.566 ↗
38. 10.1002/adhm.20240083010.1002/adhm.202400830 ↗
39. 10.1002/adma.20160684310.1002/adma.201606843 ↗
40. 10.1073/pnas.89.19.895310.1073/pnas.89.19.8953 ↗
41. 10.1021/acs.langmuir.5c0056910.1021/acs.langmuir.5c00569 ↗
42. 10.1038/s41592-020-01018-x10.1038/s41592-020-01018-x ↗
43. 10.1038/s41592-022-01663-410.1038/s41592-022-01663-4 ↗
44. 10.1016/j.ijheatmasstransfer.2020.12041210.1016/j.ijheatmasstransfer.2020.120412 ↗
45. 10.1002/smll.20240337610.1002/smll.202403376 ↗
46. 10.1021/jacs.4c0938910.1021/jacs.4c09389 ↗
47. 10.1021/acs.langmuir.7b0324310.1021/acs.langmuir.7b03243 ↗
48. 10.1016/j.eurpolymj.2021.11097410.1016/j.eurpolymj.2021.110974 ↗
49. 10.1021/acsnano.1c1022110.1021/acsnano.1c10221 ↗
50. 10.1186/s40824-023-00356-z10.1186/s40824-023-00356-z ↗
51. 10.34133/bmr.003710.34133/bmr.0037 ↗
52. 10.21769/BioProtoc.32310.21769/BioProtoc.323 ↗
53. 10.1038/s41590-024-02071-w10.1038/s41590-024-02071-w ↗
54. 10.1186/s12865-022-00505-410.1186/s12865-022-00505-4 ↗
55. 10.1126/sciadv.adu417210.1126/sciadv.adu4172 ↗
56. 10.1021/acsami.3c1126010.1021/acsami.3c11260 ↗
57. 10.3390/cancers1304087410.3390/cancers13040874 ↗
58. 10.1016/j.ces.2021.11663210.1016/j.ces.2021.116632 ↗
59. 10.1016/j.neulet.2021.13562810.1016/j.neulet.2021.135628 ↗