DataVXACS Applied Materials & InterfacesPaper
journal article·2024-07-24Open access

Surface-Charge Tuned Polymeric Nanoemulsions for Carvacrol Delivery in Interkingdom Biofilms

Jungmi Park, Zhenting Xiang, Yuan Liu, Cheng-Hsuan Li, Chider Chen, Harini Nagaraj, Tiffany Nguyen, Ahmed Nabawy, Hyun Koo, Vincent M. Rotello

ACS Applied Materials & Interfaces· Volume 16· Issue 29
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Source abstract

Abstract

Biofilms, intricate microbial communities entrenched in extracellular polymeric substance (EPS) matrices, pose formidable challenges in infectious disease treatment, especially in the context of interkingdom biofilms prevalent in the oral environment. This study investigates the potential of carvacrol-loaded biodegradable nanoemulsions (NEs) with systematically varied surface charges─cationic guanidinium (GMT-NE) and anionic carboxylate (CMT-NE). Zeta potentials of +25 mV (GMT-NE) and −33 mV (CMT-NE) underscore successful nanoemulsion fabrication (∼250 nm). Fluorescent labeling and dynamic tracking across three dimensions expose GMT-NE’s superior diffusion into oral biofilms, yielding a robust antimicrobial effect with 99.99% killing for both streptococcal and Candida species and marked reductions in bacterial cell viability compared to CMT-NE (∼4-log reduction). Oral mucosa tissue cultures affirm the biocompatibility of both NEs with no morphological or structural changes, showcasing their potential for combating intractable biofilm infections in oral environment. This study advances our understanding of NE surface charges and their interactions within interkingdom biofilms, providing insights crucial for addressing complex infections involving bacteria and fungi in the demanding oral context.
Source: openalex_inverted_index · Quality: baseline

Research context

PolymersNanomaterialsBiomaterials

OpenAlex topics: Bone Tissue Engineering Materials · Polymer Surface Interaction Studies · Electrophoretic Deposition in Materials Science

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, Massachusetts 01003, United States

Biofilm Research Laboratories, Levy Center for Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States

Department of Orthodontics, School of Dental Medicine, University of Pennsylvania, 240 S 40th Street, Philadelphia, Pennsylvania 19104, United States

Department of Preventive and Restorative Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States

Department of Oral and Maxillofacial Surgery and Pharmacology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States

Center for Innovation and Precision Dentistry, School of Dental Medicine, School of Engineering and Applied Sciences, University of Pennsylvania, 240 S 40th Street, Philadelphia, Pennsylvania 19104, United States

Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, 210 S 33rd Street, Philadelphia, Pennsylvania 19104, United States

Funding

Colgate-Palmolive Company · National Institute of Dental and Craniofacial Research · National Institute of Allergy and Infectious Diseases

References (57)

Show source-provided references
1. 10.1186/s13756-019-0533-310.1186/s13756-019-0533-3 ↗
2. 10.3389/fcimb.2023.113794710.3389/fcimb.2023.1137947 ↗
3. 10.1038/s41579-022-00791-010.1038/s41579-022-00791-0 ↗
4. 10.1016/j.tim.2011.07.00410.1016/j.tim.2011.07.004 ↗
5. 10.1038/s41396-018-0113-110.1038/s41396-018-0113-1 ↗
6. 10.3390/pathogens1002014410.3390/pathogens10020144 ↗
7. 10.1128/AAC.00657-0910.1128/AAC.00657-09 ↗
8. 10.3390/jof503008110.3390/jof5030081 ↗
9. 10.3389/fmicb.2015.0066910.3389/fmicb.2015.00669 ↗
10. 10.1038/s41579-022-00720-110.1038/s41579-022-00720-1 ↗
11. Allison, D. L.; Willems, H. M. E.; Jayatilake, J. A. M. S.; Bruno, V. M.; Peters, B. M.; Shirtliff, M. E. Candida-Bacteria Interactions: Their Impact on Human Disease; Kudva, I. T.; Plummer, P. J.; Allison, D. L.; Willems, H.; Jayatilake, J.; Bruno, V. M.; Peters, B. M.; Shirtliff, M. E., Eds. Wiley, 2016; Vol. 4, pp. 1-26.10.1128/microbiolspec.VMBF-0030-2016 ↗
12. 10.1177/002203451879094110.1177/0022034518790941 ↗
13. 10.1016/j.tim.2023.08.00310.1016/j.tim.2023.08.003 ↗
14. 10.3389/fcimb.2023.110623110.3389/fcimb.2023.1106231 ↗
15. 10.1128/IAI.00087-1410.1128/IAI.00087-14 ↗
16. 10.1021/acsinfecdis.9b0013410.1021/acsinfecdis.9b00134 ↗
17. 10.1016/j.phymed.2011.06.03310.1016/j.phymed.2011.06.033 ↗
18. 10.3390/molecules2420371310.3390/molecules24203713 ↗
19. 10.1002/ptr.610310.1002/ptr.6103 ↗
20. 10.1111/ics.1276110.1111/ics.12761 ↗
21. 10.1039/b821648g10.1039/b821648g ↗
22. 10.3390/antibiotics1205082410.3390/antibiotics12050824 ↗
23. 10.1007/s10096-010-1050-810.1007/s10096-010-1050-8 ↗
24. 10.1016/j.biopha.2021.11221810.1016/j.biopha.2021.112218 ↗
25. 10.1016/j.sjbs.2021.05.03310.1016/j.sjbs.2021.05.033 ↗
26. 10.3390/antibiotics1208124010.3390/antibiotics12081240 ↗
27. 10.1038/s41579-020-0420-110.1038/s41579-020-0420-1 ↗
28. 10.3390/nano1011225310.3390/nano10112253 ↗
29. 10.1038/s41579-020-0420-110.1038/s41579-020-0420-1 ↗
30. 10.1039/D1SC05835E10.1039/D1SC05835E ↗
31. 10.1002/macp.20220022610.1002/macp.202200226 ↗
32. 10.1021/jacs.8b0357510.1021/jacs.8b03575 ↗
33. 10.1016/S0167-7012(00)00224-410.1016/S0167-7012(00)00224-4 ↗
34. 10.3389/fmicb.2019.0299310.3389/fmicb.2019.02993 ↗
35. 10.3390/microorganisms902041210.3390/microorganisms9020412 ↗
36. 10.1016/j.bbamem.2021.18359710.1016/j.bbamem.2021.183597 ↗
37. 10.4103/0972-124X.10747310.4103/0972-124X.107473 ↗
38. 10.1016/j.polymer.2013.03.02710.1016/j.polymer.2013.03.027 ↗
39. 10.1038/ncomms621910.1038/ncomms6219 ↗
40. 10.1038/nrmicro.2017.9910.1038/nrmicro.2017.99 ↗
41. 10.1371/journal.ppat.100734210.1371/journal.ppat.1007342 ↗
42. 10.1039/C7CS00807D10.1039/C7CS00807D ↗
43. 10.3389/fcimb.2015.0001010.3389/fcimb.2015.00010 ↗
44. 10.1016/S0927-7765(99)00035-110.1016/S0927-7765(99)00035-1 ↗
45. 10.1006/emyc.1994.101310.1006/emyc.1994.1013 ↗
46. 10.1021/acsbiomaterials.9b0006910.1021/acsbiomaterials.9b00069 ↗
47. 10.1039/C4CC07737G10.1039/C4CC07737G ↗
48. 10.1021/acsinfecdis.5b0011610.1021/acsinfecdis.5b00116 ↗
49. 10.1007/s00253-012-4091-310.1007/s00253-012-4091-3 ↗
50. 10.1128/AEM.05203-1110.1128/AEM.05203-11 ↗
51. 10.1371/journal.ppat.100262310.1371/journal.ppat.1002623 ↗
52. 10.1016/j.archoralbio.2006.06.00110.1016/j.archoralbio.2006.06.001 ↗
53. 10.1038/s41467-018-05342-x10.1038/s41467-018-05342-x ↗
54. 10.1016/j.jconrel.2022.05.01310.1016/j.jconrel.2022.05.013 ↗
55. 10.1038/nrm387310.1038/nrm3873 ↗
56. 10.3390/ijms2307403810.3390/ijms23074038 ↗
57. 10.1007/s10439-016-1594-610.1007/s10439-016-1594-6 ↗