DataVXACS Applied Materials & InterfacesPaper
journal article·2023-04-19Open access

Preservation of Proteins in Human Plasma through Metal–Organic Framework Encapsulation

Yixuan Wang, Jeremiah J. Morrissey, Prashant Gupta, Pradeep Chauhan, Russell K. Pachynski, Peter K. Harris, Aadel Chaudhuri, Srikanth Singamaneni

ACS Applied Materials & Interfaces· Volume 15· Issue 15
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Source abstract

Abstract

Traditional cold chain systems of collection, transportation, and storage of biofluid specimens for eventual analysis pose a huge financial and environmental burden. These systems are impractical in pre-hospital and resource-limited settings, where refrigeration and electricity are not reliable or even available. Here, we develop an innovative technology using metal-organic frameworks (MOFs), a novel class of organic-inorganic hybrids with high thermal stability, as encapsulates for preserving the integrity of protein biomarkers in biofluids under ambient or non-refrigerated storage conditions. We encapsulate prostate-specific antigen (PSA) in whole patient plasma using hydrophilic zeolitic imidazolate framework-90 (ZIF-90) for preservation at 40 °C for 4 weeks and eventual on-demand reconstitution for antibody-based assays with recovery above 95% compared to storage at -20 °C. Without ZIF-90 encapsulation, only 10-30% of the PSA immunoactivity remained. Furthermore, we demonstrate encapsulation of multiple cancer biomarker proteins in whole patient plasma using ZIF-8 or ZIF-90 encapsulants for eventual on-demand reconstitution and analysis after 1 week at 40 °C. Overall, MOF encapsulation of patient biofluids is important as climate change may be affecting the stability and increase costs of maintaining biospecimen cold chain custody for the collection, transportation, and storage of biospecimens prior to analysis or for biobanking regardless of any countries' affluence.
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Research context

OpenAlex topics: Metal-Organic Frameworks: Synthesis and Applications · Mass Spectrometry Techniques and Applications · Machine Learning in Materials Science

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Mechanical Engineering and Materials Science, Institute of Materials Science and Engineering, Washington University in St. Louis, St Louis, Missouri 63130, United States

Department of Anesthesiology, Division of Clinical and Translational Research, Washington University School of Medicine in St. Louis, St Louis, Missouri 63110, United States

Siteman Cancer Center, Barnes-Jewish Hospital and Washington University School of Medicine in St. Louis, St Louis, Missouri 63110, United States

Division of Cancer Biology, Department of Radiation Oncology, Washington University School of Medicine in St. Louis, St Louis, Missouri 63110, United States

Division of Oncology, Department of Medicine, Washington University School of Medicine in St. Louis, St Louis, Missouri 63110, United States

Department of Biomedical Engineering, Washington University School of Medicine in St. Louis, St Louis, Missouri 63110, United States

Department of Genetics, Washington University School of Medicine in St. Louis, St Louis, Missouri 63110, United States

Department of Computer Science and Engineering, Washington University in St. Louis, St Louis, Missouri 63130, United States

Funding

National Cancer Institute

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