SPECT/CT Imaging of ¹¹¹Ag for the Preclinical Evaluation of Silver-Based Antimicrobial Nanomedicines

Colin Blackadar; Ka-Yee Grace Choi; Mary F. Embree; Heather M. Hennkens; Cristina Rodríguez-Rodríguez; Robert E. W. Hancock; Katayoun Saatchi; Urs O. Häfeli

doi:10.1021/acsami.2c03609

journal article·2022-06-15

SPECT/CT Imaging of ¹¹¹Ag for the Preclinical Evaluation of Silver-Based Antimicrobial Nanomedicines

Colin Blackadar, Ka-Yee Grace Choi, Mary F. Embree, Heather M. Hennkens, Cristina Rodríguez-Rodríguez, Robert E. W. Hancock, Katayoun Saatchi, Urs O. Häfeli

ACS Applied Materials & Interfaces· Volume 14· Issue 23

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Abstract

With the growing interest in developing silver-based antimicrobials, there is a need to better understand the behavior of silver within biological systems. To address this, we showed that single-photon emission computed tomography (SPECT) is a suitable method to noninvasively image 111Ag-labeled compounds in mice. Formed by neutron irradiation of palladium foil, 111Ag can be rapidly isolated with a high degree of purity and stably incorporated into antimicrobial silver nanoparticles. The imaging showed that nanoparticles are retained in the lungs for up to 48 h following intratracheal instillation, with limited uptake into the systemic circulation or organs of the reticuloendothelial system. Furthermore, in a mouse model of pulmonary Pseudomonas aeruginosa infection, the nanoparticles reduced the bacterial burden by 11.6-fold without inducing the production of pro-inflammatory mediators. Overall, SPECT imaging with 111Ag is a useful tool for noninvasively visualizing the biodistribution of silver-containing compounds in rodents. This knowledge of how silver nanoparticles distribute in vivo can be used to predict their therapeutic efficacy.

Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Nanoparticles: synthesis and applications · Nanoparticle-Based Drug Delivery · Orthopedic Infections and Treatments

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Faculty of Pharmaceutical Sciences, University of British Columbia, 2405 Wesbrook Mall, Vancouver, BC V6T1Z3, Canada

Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, Faculty of Science, University of British Columbia, Vancouver, BC V6T1Z4, Canada

University of Missouri Research Reactor Center (MURR), 13513 Research Park Drive, Columbia, Missouri 65211, United States

Department of Chemistry, University of Missouri, 125 Chemistry Building, Columbia, Missouri 65211, United States

Department of Physics and Astronomy, University of British Columbia, 6224 Agricultural Road, Vancouver, BC V6T1Z1, Canada

Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, Copenhagen 2100, Denmark

Funding

Canadian Institutes of Health Research · Lotte and John Hecht Memorial Foundation · Canada Foundation for Innovation · Canada Research Chairs · Natural Sciences and Engineering Research Council of Canada · Michael Smith Foundation for Health Research · Lundbeckfonden

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