DataVXACS Applied Materials & InterfacesPaper
journal article·2023-06-14

High-Strength Cell Sheets and Vigorous Hydrogels from Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells

Baojie Guo, Yongchao Duan, Zhongwen Li, Yao Tian, Xuedi Cheng, Chunxu Liang, Wenjing Liu, Bin An, Wumei Wei, Tingting Gao, Shasha Liu, Xiyuan Zhao, Shuaishuai Niu, Chenxin Wang, Yukai Wang, Liu Wang, Guihai Feng, Wei Li, Jie Hao, Qi Gu, Qi Zhou, Jun Wu

ACS Applied Materials & Interfaces· Volume 15· Issue 23
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Source abstract

Abstract

Natural cell derivates, including cell sheets (CSs) and matrix gels, have opened new opportunities to probe questions in tissue engineering and regenerative medicine. However, the potential of CSs and hydrogels generated by current protocols is still limited by the challenges of heterogeneity and weak mechanical properties. Here, we developed a 21 day long-term serum-free culture system for human embryonic stem cell (hESC)-derived immunity-and-matrix-regulatory cells (IMRCs). The CSs formed with IMRCs (IMRC-CSs) have a much greater secretion capacity for the extracellular matrix (ECM) and stronger mechanical properties than umbilical cord-derived MSCs, with a ten thousand-fold increase in elastin, a higher elastic modulus of 1500 kPa, a thicker structure of 20.59 μm, and a higher fiber count per square millimeter. The IMRC-CSs could promote corneal chemical injury repair and could be turned into injectable temperature-sensitive hydrogels for uterine adhesion repair via a decellularization process. In summary, we have established a high-strength CS platform using human pluripotent stem cells for the first time, providing a facile and scalable engineering approach for regenerative medicine.
Source: openalex_inverted_index · Quality: baseline

Research context

PolymersBiomaterials

OpenAlex topics: Tissue Engineering and Regenerative Medicine · Corneal Surgery and Treatments · Electrospun Nanofibers in Biomedical Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China

University of Chinese Academy of Sciences, Beijing 100049, China

Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China

College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering and Technology, Northwest A&F University, Xianyang, Shanxi 712100, China

Agriculture College, Yanbian University, Jilin 133002, China

National Stem Cell Resource Center, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Funding

National Key Research and Development Program of China · National Natural Science Foundation of China · Chinese Academy of Sciences · Chinese Academy of Sciences Engineering Laboratory for Intelligent Organ Manufacturing · China Manned Space Program

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