Rapid and High-Density Antibody Immobilization Using Electropolymerization of Pyrrole for Highly Sensitive Immunoassay
Source abstract
Abstract
Polypyrrole (Ppy) is a biologically compatible polymer that is used as a matrix, in which drugs and enzymes can be incorporated by doping. Here, we suggest an inventive application of Ppy as a biorecognition film encapsulated with an antibody (Ab) as an alternative strategy for the on-site multistep functionalization of thiol-based self-assembled monolayers. The fabrication steps of the recognition films were followed by dropping pyrrole and Ab mixed solutions onto the electrode and obtaining a thin film by direct current electropolymerization. The efficiency of Ab immobilization was studied by using fluorescence microscopy and electrochemical (EC) methods. Finally, the Ab density was increased and immobilized in 1 min, and the sensing performance as an EC immunosensor was demonstrated using α-fetoprotein with a limit of detection of 3.13 pg/mL and sensing range from 1 pg/mL to 100 ng/mL. This study demonstrates the potential for electrochemical functionalization of biomolecules with high affinity and rapidity.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Molecular Junctions and Nanostructures · Advanced biosensing and bioanalysis techniques · Electrochemical sensors and biosensors
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Medical IT Convergence, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, Republic of Korea
Korea Institute of Toxicology (KIT), Jeongeup, Jeollabuk-do 56212, Republic of Korea
Digital Healthcare Research Center, Gumi Electronics and Information Technology Research Institute (GERI), Gumi, Gyeongbuk 39253, Republic of Korea
Department of Dermatology, School of Medicine, University of Washington, 850 Republican Street, Seattle, Washington 98109, United States
Institute for Stem Cell and Regenerative Medicine, University of Washington, 850 Republican Street, Seattle, Washington 98109, United States
The Laboratory of Viromics and Evolution, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan-si, Jeollabuk-do 54531, Republic of Korea
Funding
Elsa U. Pardee Foundation · National Research Foundation of Korea · Korea Technology and Information Promotion Agency for SMEs
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